Stability of interleukin 8 and neutrophil elastase in bronchoalveolar lavage fluid following long-term storage

Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
Luke J BerryAREST CF

Abstract

Interleukin-8 (IL-8) and neutrophil elastase (NE) are commonly measured markers of inflammation in bronchoalveolar lavage (BAL) fluid from patients with cystic fibrosis. Longitudinal analysis assumes uniform stability during storage, however the effect of extended low-temperature storage on these markers remains unclear. BAL fluid from 104 children with cystic fibrosis was assayed for IL-8 and NE after storage at 4 ° C for 7 days and -80 ° C for up to 6 years and compared with the initial assays performed soon after collection. IL-8 levels were stable after any measured length of time at -80 ° C or 4 ° C. NE levels were stable for 6 months at -80 ° C but decreased beyond that or after 7 days at 4 ° C. Our data support the stability of IL-8 in BAL stored at -80 ° C for prolonged periods. NE in BAL decreases with storage and should be assayed as soon as practical after collection.

References

Jan 6, 2001·Journal of Clinical Periodontology·J M HerrmannJ Meyle
Feb 28, 2002·The European Respiratory Journal·V De Rose
Feb 18, 2006·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·J A FillB G Zeiher
Jul 26, 2006·Mediators of Inflammation·Anna ZalewskaAnna Sysa-Jedrzejowska
Apr 24, 2007·Cytokine·Gitika PanickerElizabeth R Unger
Apr 9, 2008·The International Journal of Biochemistry & Cell Biology·Judith A VoynowShuo Zheng
Apr 18, 2009·American Journal of Respiratory and Critical Care Medicine·Peter D SlyUNKNOWN Australian Respiratory Early Surveillance Team for Cystic Fibrosis (AREST-CF)
Oct 3, 2009·The European Respiratory Journal·S BielsaY C G Lee

❮ Previous
Next ❯

Citations

Sep 1, 2011·Biopreservation and Biobanking·Allison HubelXiao Zhong
Jul 30, 2015·American Journal of Respiratory Cell and Molecular Biology·Luke W GarrattUNKNOWN AREST CF
Sep 20, 2012·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Natalie BeiersdorfJochen G Mainz
Oct 8, 2014·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Julia HentschelJochen G Mainz
May 2, 2015·The European Respiratory Journal·Luke W GarrattUNKNOWN Australian Respiratory Early Surveillance Team for Cystic Fibrosis (AREST CF)
Apr 22, 2018·The European Respiratory Journal·Oded BreuerUNKNOWN AREST CF
Feb 28, 2019·American Journal of Respiratory and Critical Care Medicine·Oded BreuerDaan Caudri

❮ Previous
Next ❯

Related Concepts

Related Feeds

CFTR Mutant Structural Therapy

Over 1700 different mutations in the CFTR genes have been shown to cause cystic fibrosis. Here is the latest research on structural therapy for CFTR mutants.

Related Papers

Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
Cyrille BergoinBenoît Wallaert
American Journal of Obstetrics and Gynecology
C Y SpongJ C Pezzullo
Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
Natalie BeiersdorfJochen G Mainz
© 2021 Meta ULC. All rights reserved