Stability of Phase II Conjugation Systems in Cultured Precision-cut Rat Hepatic Slices

Toxicology in Vitro : an International Journal Published in Association with BIBRA
E HashemiCostas Ioannides

Abstract

The aim of the present study was to investigate the stability of Phase II conjugation enzymes in precision-cut rat liver slices that have been cultured, using the multiwell plate system, for various periods of time up to 72 hours. The enzyme activities monitored were epoxide hydrolase, glutathione S-transferase and the associated glutathione reductase, sulfotransferase and glucuronyl transferase. The activity of all enzymes studied declined with time when expressed per slice, but the rate of loss differed among the various enzymes. However, in most cases substantial Phase II activity was still present in the liver slices following a 24-hour incubation, and in some cases significant activity was even retained in slices cultured for 72 hours. These studies indicate that precision-cut liver slices maintain Phase II activity for long periods of time, thus allowing metabolic studies involving prolonged incubations to be performed.

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Citations

Nov 25, 2000·Toxicology·C Lerche-Langrand, H J Toutain
Nov 8, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E EvdokimovaP Buc Calderon
Jan 29, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E EvdokimovaP Buc Calderon
Aug 22, 2007·Tissue Engineering·Yuet Mei KhongHanry Yu
Apr 19, 2007·Clinical and Experimental Pharmacology & Physiology·Yu GuoChun Zhang
Oct 31, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Viktoriia StarokozhkoGeny M M Groothuis
Apr 21, 2004·Journal of Veterinary Pharmacology and Therapeutics·S SivapathasundaramC Ioannides
Jan 29, 2011·Biotechnology and Bioengineering·Paul M van MidwoudElisabeth Verpoorte
Jul 25, 2003·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Klaus PissowotzkiDieter Müller
Jun 30, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Costas Ioannides
Mar 16, 2013·Toxicology Research·Valerie Y SoldatowIvan Rusyn
Feb 5, 2005·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M UmachandranC Ioannides

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