Stability of platelet activating factor (PAF) in human saliva. Quantitation by radioimmunoassay

Clinica Chimica Acta; International Journal of Clinical Chemistry
S J CooneyB A Baldo

Abstract

Platelet activating factor (PAF) is thought to mediate many inflammatory processes and its involvement in health and disease may be clarified by examining PAF levels in human secretions. The known presence of PAF, the ease of obtaining samples and the relative stability of PAF in saliva, makes this fluid a preferred source for examination of PAF in health and disease. The activity of PAF-acetylhydrolase (the PAF degrading enzyme) in saliva was 1,000-fold lower than that found in human plasma. Extraction of saliva with chloroform/methanol/water resulted in 70-90% recovery of PAF. Using the radioimmunoassay (RIA), PAF levels in the range 0.5-21 ng/ml were found in normal human salivas. These values were significantly higher than those reported from bioassay studies based on washed platelets. The validity of the RIA was checked by isolating and quantitating the PAF fraction from whole saliva extract, and by treatment of the extracts with the enzyme phospholipase A2. Direct comparison of salivary PAF levels, determined by both platelet aggregation (PA) and RIA confirmed our original finding that values obtained were lower using the bioassay method. Furthermore, these bioassay values compared favourably with those in the literature....Continue Reading

References

Apr 17, 1990·Journal of Immunological Methods·M A SmalA McCaskill
Apr 1, 1989·Biomedical & Environmental Mass Spectrometry·B W Christman, I A Blair
Aug 1, 1989·Molecular Immunology·M A SmalJ W Redmond
Apr 1, 1987·British Medical Bulletin·B B Vargaftig, P G Braquet
Jan 1, 1986·Annual Review of Biochemistry·D J Hanahan
Jan 1, 1985·Methods of Biochemical Analysis·D J Hanahan, S T Weintraub
Jan 1, 1983·Archives of Oral Biology·B L SlomianyI D Mandel
Oct 1, 1982·Journal of Dental Research·B L SlomianyL A Tabak
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Aug 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steffen HelmlingSven Klussmann

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Citations

Aug 5, 1998·Journal of Periodontal Research·E RibaldiP Gresele
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·L M McManus, R N Pinckard
Apr 1, 1995·Journal of Dental Research·M L GaritoL M McManus

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