Stability of protein and ribonucleic acid in Bacillus stearothermophilus

Journal of General Microbiology
T P CoultateJ J Cazzulo

Abstract

The turnover of protein in a prototrophic strain of Bacillus stearothermophilus during exponential growth in a salts medium with glucose or succinate as carbon source was about 4 %/h and in a richer nutrient broth medium about 23 %/h. Protein degradation under non-growing conditions conformed to a similar pattern. The turnover of RNA (non-messenger) was about 1 %/h in salts medium and about 9 %/h in nutrient broth. The turnover of protein and RNA in the thermophile is thus moderate rather than massive. This conclusion was confirmed by measurement of the decay of a specific enzyme, isocitrate lyase, in the prototroph and of the overall protein turnover in a non-prototrophic strain of B. stearothermophilus. The half-lives of a number of enzyme systems in intact cells of the prototrophic thermophile at its optimum growth temperature showed some variation but indicated a significant rate of inactivation. Such decay of protein in vivo apparently accounts for the moderate protein turnover observed during growth.

Citations

Dec 1, 1980·Archives of Microbiology·P J RogersA F Egan
Jan 1, 1978·CRC Critical Reviews in Microbiology·R E Amelunxen, A L Murdock
Jun 6, 1997·Journal of Toxicology and Environmental Health·I al-Saleh, I al-Doush
Nov 22, 1979·Biochimica Et Biophysica Acta·J Stenesh, J B Madison

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