Stability of Proteins in Dried Blood Spot Biobanks.

Molecular & Cellular Proteomics : MCP
Johan BjörkestenUlf Landegren

Abstract

An important motivation for the construction of biobanks is to discover biomarkers that identify diseases at early, potentially curable stages. This will require biobanks from large numbers of individuals, preferably sampled repeatedly, where the samples are collected and stored under conditions that preserve potential biomarkers. Dried blood samples are attractive for biobanking because of the ease and low cost of collection and storage. Here we have investigated their suitability for protein measurements. Ninety-two proteins with relevance for oncology were analyzed using multiplex proximity extension assays (PEA) in dried blood spots collected on paper and stored for up to 30 years at either +4 °C or -24 °C.Our main findings were that (1) the act of drying only slightly influenced detection of blood proteins (average correlation of 0.970), and in a reproducible manner (correlation of 0.999), (2) detection of some proteins was not significantly affected by storage over the full range of three decades (34 and 76% of the analyzed proteins at +4 °C and -24 °C, respectively), whereas levels of others decreased slowly during storage with half-lives in the range of 10 to 50 years, and (3) detectability of proteins was less affected...Continue Reading

References

Nov 1, 1989·Pharmaceutical Research·M C ManningR T Borchardt
Dec 24, 1997·Molecular Biotechnology·D B VolkinC R Middaugh
Jun 27, 2000·Journal of Biotechnology·R Jaenicke
Jul 27, 2001·Current Pharmaceutical Biotechnology·Y F Maa, S J Prestrelski
Apr 23, 2008·Current Protocols in Protein Science·John F CarpenterTheodore W Randolph
Oct 30, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Fredrika GauffinHåkan Karlsson
Oct 7, 2011·Clinical Microbiology Reviews·Shajo Kunnath-Velayudhan, Maria Laura Gennaro
Jan 17, 2012·American Journal of Human Genetics·Peter M VisscherJian Yang
Feb 20, 2013·Nature Reviews. Rheumatology·William H RobinsonJeremy Sokolove
Jun 4, 2013·Journal of the American Society for Mass Spectrometry·Nicholas J MartinHelen J Cooper
Jul 5, 2013·Lab on a Chip·Maïwenn Kersaudy-Kerhoas, Elodie Sollier
Jul 24, 2013·Plant Methods·Arthur Korte, Ashley Farlow
Sep 24, 2013·Lab on a Chip·Stefano BegoloRustem F Ismagilov
Oct 26, 2013·Analytical Chemistry·Jin-Hee KimFred E Regnier
Sep 11, 2014·Clinical Pharmacokinetics·Abraham J WilhelmEleonora L Swart
Sep 16, 2014·Proteomics. Clinical Applications·Vera IgnjatovicJeffrey M Craig
Jan 1, 2013·Personalized Medicine·Mauricio FloresLeroy Hood
Apr 14, 2015·Journal of Visualized Experiments : JoVE·Nico GrünerR Stefan Ross
Jun 17, 2015·Journal of Immunological Methods·Anders LarssonMasood Kamali-Moghaddam
Sep 4, 2015·Bioanalysis·Gabriel LenkNiclas Roxhed
Sep 6, 2015·Molecular & Cellular Proteomics : MCP·Andrew G ChambersChristoph H Borchers
Jan 23, 2016·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Valentina BertiAlberto Pupi
Aug 10, 2016·Lab on a Chip·W S MielczarekM Kersaudy-Kerhoas

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Citations

Jul 4, 2018·The American Journal of Tropical Medicine and Hygiene·Mark D Lim
Sep 6, 2017·Omics : a Journal of Integrative Biology·Jae-Eun Lee, Young-Youl Kim
Jun 23, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ayman AlhamdowKarin Broberg
Aug 15, 2019·Glycobiology·Jelena SimunovicMarija Pezer
Sep 7, 2019·Mass Spectrometry Reviews·Benjamin S FreyAbraham K Badu-Tawiah
Feb 21, 2019·Analytical and Bioanalytical Chemistry·Yukiko YanoStephen Rappaport
Sep 21, 2018·Gates Open Research·Malia S Q MurphyKumanan Wilson
Sep 8, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Alan LevitonOlaf Dammann
Oct 9, 2019·Therapeutic Drug Monitoring·Laura E J PeetersBirgit C P Koch
Dec 21, 2018·International Journal of Molecular Sciences·Éva CsőszJózsef Tőzsér
Feb 23, 2018·Expert Review of Proteomics·Rhian Lauren PreeceSabine Bahn
Oct 27, 2020·The Pediatric Infectious Disease Journal·Feimei LiuShelli F Farhadian
Oct 5, 2020·Molecular Oncology·Ulf Landegren, Maria Hammond
Nov 17, 2020·Drug Testing and Analysis·Gemma Reverter-BranchatOscar J Pozo
Feb 11, 2021·Biopreservation and Biobanking·David CarpentieriT A Sivakumaran
Jan 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Camilla MarascaAndrea Armirotti
Jul 3, 2021·Autism Research : Official Journal of the International Society for Autism Research·Renee M GardnerHåkan Karlsson
Oct 14, 2021·Cell Death & Disease·Mahsa Shahidi DadrasAristidis Moustakas
Jan 9, 2018·New Biotechnology·Ulf LandegrenJohan Björkesten

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Methods Mentioned

BETA
PCR
chip

Software Mentioned

R Development Core Team
R

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