PMID: 2496112May 1, 1989Paper

Stability of reiterated sequences in the Bacillus subtilis chromosome

Journal of Bacteriology
M Young, S D Ehrlich

Abstract

The instability of reiterated sequences in the Bacillus subtilis chromosome that was previously reported (M. Young, J. Gen. Microbiol. 130:1613-1621, 1984) results from the presence of a truncated pC194 replication origin together with an intact replication protein A gene in the amplified DNA. Removal of the truncated pC194 replication origin or inactivation of replication protein A stabilizes reiterated sequences, whereas provision of replication protein A in trans destabilizes them. We suggest that residual activity of protein A at the truncated replication origin generates single-stranded DNA, which stimulates recombination between repeated sequences and thus destabilizes amplified structures.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Mar 25, 1988·Cell·D BrunierS D Ehrlich
Aug 1, 1985·Journal of Bacteriology·C R Wilson, A E Morgan
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H te RieleS D Ehrlich
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Jun 1, 1983·Journal of Bacteriology·F A FerrariJ A Hoch
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Citations

Mar 1, 1992·Molecular Microbiology·S KamounC I Kado
Jan 1, 1996·Applied Biochemistry and Biotechnology·E M Green, G N Bennett
Jan 1, 1995·FEMS Microbiology Letters·M TangneyS T Jørgensen
Jan 1, 1997·Annual Review of Genetics·D Romero, R Palacios
Dec 2, 2000·Microbiology·K C JennertM Young
Sep 1, 1991·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Sep 1, 1991·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Sep 1, 1990·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Mar 1, 1990·Gene·L JannièreS D Ehrlich
Aug 1, 1990·Journal of Bacteriology·R Gopal-SrivastavaP B Hylemon

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