Stability of Routine Biochemical Analytes in Whole Blood and Plasma From Lithium Heparin Gel Tubes During 6-hr Storage

Journal of Clinical Laboratory Analysis
Denis MonneretDominique Bonnefont-Rousselot

Abstract

The stability of biochemical analytes has already been investigated, but results strongly differ depending on parameters, methodologies, and sample storage times. We investigated the stability for many biochemical parameters after different storage times of both whole blood and plasma, in order to define acceptable pre- and postcentrifugation delays in hospital laboratories. Twenty-four analytes were measured (Modular® Roche analyzer) in plasma obtained from blood collected into lithium heparin gel tubes, after 2-6 hr of storage at room temperature either before (n = 28: stability in whole blood) or after (n = 21: stability in plasma) centrifugation. Variations in concentrations were expressed as mean bias from baseline, using the analytical change limit (ACL%) or the reference change value (RCV%) as acceptance limit. In tubes stored before centrifugation, mean plasma concentrations significantly decreased after 3 hr for phosphorus (-6.1% [95% CI: -7.4 to -4.7%]; ACL 4.62%) and lactate dehydrogenase (LDH; -5.7% [95% CI: -7.4 to -4.1%]; ACL 5.17%), and slightly decreased after 6 hr for potassium (-2.9% [95% CI: -5.3 to -0.5%]; ACL 4.13%). In plasma stored after centrifugation, mean concentrations decreased after 6 hr for bicarbo...Continue Reading

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Citations

Sep 10, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Anne Marie DupuyStéphanie Badiou
Aug 24, 2018·Diagnosis·Kjell GrankvistAlexander von Meyer
Apr 14, 2020·Scandinavian Journal of Clinical and Laboratory Investigation·Lieselot CoolKoen Van Eygen
Sep 2, 2020·Journal of Clinical Laboratory Analysis·Mehdi HedayatiSima Kheradmand Kia
May 8, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Rubén Gómez RiojaVirtudes Álvarez
Feb 28, 2019·Diagnosis·Mads NyboKjell Grankvist
Jul 28, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Rubén Gómez-RiojaUNKNOWN Analytical Quality Commission of the Spanish Society of Laboratory Medicine (SEQCML)
Oct 28, 2019·Practical Laboratory Medicine·M J A J HuijskensF M Verheijen
Jun 22, 2021·American Journal of Clinical Pathology·Megan E DibbernJoesph R Wiencek
Nov 3, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Kobe VercruysseMatthijs Oyaert
Feb 4, 2022·Scandinavian Journal of Clinical and Laboratory Investigation·Agnes Ziobrowska-BechPeter Astrup Christensen

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