Stability of S-HBsAg in long-term stored lyophilised plant tissue

Biologicals : Journal of the International Association of Biological Standardization
Marcin CzyżTomasz Pniewski

Abstract

A potent plant-derived oral vaccine against Hepatitis B Virus (HBV) requires a durable and compact form for efficacious and convenient distribution and delivery. In the previous study we have devised a successful freeze-drying process of plant material containing the HBV small surface antigen (S-HBsAg) for the purpose of an oral vaccine against the virus, but product storage stability was limited to 4 °C. The aim of this study was to upgrade a freeze-dried product formula to facilitate successful long-term storage of S-HBsAg assembled into Virus-Like Particles (VLPs) at elevated temperatures. Series of additional excipients and storage conditions were tested. Atmosphere of nitrogen proved to preserve S-HBsAg VLPs most efficiently, with only minor degradation at the highest temperature of 37 °C. As a result, a semi-product for the oral plant-derived vaccine against HBV with good storage capabilities was obtained.

References

May 7, 1999·Journal of Pharmaceutical Sciences·M C Lai, E M Topp
Sep 1, 2000·International Journal of Pharmaceutics·W Wang
Apr 28, 2001·Advanced Drug Delivery Reviews·S R ByrnA W Newman
Dec 5, 2002·Current Pharmaceutical Biotechnology·Rajesh Krishnamurthy, Mark C Manning
Jun 4, 2004·Journal of Pharmaceutical Sciences·Yuh-Fun MaaDexiang Chen
Jan 25, 2007·Pharmaceutical Research·Dushyant B VarshneyRaj Suryanarayanan
Feb 3, 2007·Current Drug Delivery·Yuh-Fun MaaDexiang Chen
Dec 29, 2007·Pharmaceutical Development and Technology·Stéphanie PassotMichel Rapaud
Oct 18, 2012·Current Pharmaceutical Biotechnology·Tomasz Pniewski
Nov 6, 2014·BioMed Research International·Marcin CzyżTomasz Pniewski

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Citations

Oct 19, 2016·International Journal of Molecular Sciences·Young Hee JoungHyun-Soon Kim
May 8, 2018·Planta·Claudia A Espinosa-LealSilverio García-Lara
Sep 9, 2017·Future Science OA·Kathleen Hefferon

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