Mar 1, 1976

Stability of the insoluble form of uridine kinase coupled to zn2+ or pb2+ ions

Hoppe-Seyler's Zeitschrift für physiologische Chemie
A Cihák

Abstract

Partially purified calf brain uridine kinase precipitated by bivalent metal cations has been compared with the soluble enzyme fraction regarding its stability in the presence of inactivating factors. The freeze-dried preparations of uridine kinase precipitaated by Pb2+ or Zn2+ ions, althouth enzymatically highly active, are insoluble in aqueous solutions. The activity of metal-insolubilized enzymes disappears during their preincubation in acidic media or in the presence of silver ions. Also trypsin, chymotrypsin and cathepsin B1 caused decreases in enzyme activity. However, fractions which have been precipitated by metal ions and freeze-dried are stable at high temperatures, whereas the activity of soluble uridine kinase is completely lost. Both unheated metal-ion precipitated uridine kinase preparations and those heated at 100 degrees C are equally sensitive to the feedback inhibition by CTP.

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References

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Citations

Mentioned in this Paper

Structure of Calf of Leg
Azauridine
Enzymes, antithrombotic
2-Mercaptoethanol
Cattle calf (organism)
Brain
Bos taurus
Cathepsins B
Sputolysin
Feedback - System Communication

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