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Stability parameters for one-step mechanism of irreversible protein denaturation: a method based on nonlinear regression of calorimetric peaks with nonzero deltaCp

Analytical Biochemistry

May 15, 2004

Alfonso Arroyo-ReynaArturo Rojo-Domínguez

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Abstract

Thermal transitions of many proteins have been found to be calorimetrically irreversible and scan-rate dependent. Calorimetric determinations of stability parameters of proteins which unfold irreversibly according to a first-order kinetic scheme have been reported. These methods require...read more

Mentioned in this Paper

Inorganic phosphate
Phosphate ion
Proteolytic Enzyme
Natto Bacteria
Regression Analysis
Subtilisin
Thermodynamics
Scanning
Denaturation
Subtilisins
Paper Details
References
  • References20
  • Citations9
12

Stability parameters for one-step mechanism of irreversible protein denaturation: a method based on nonlinear regression of calorimetric peaks with nonzero deltaCp

Analytical Biochemistry

May 15, 2004

Alfonso Arroyo-ReynaArturo Rojo-Domínguez

PMID: 15113687

DOI: 10.1016/j.ab.2004.02.021

Abstract

Thermal transitions of many proteins have been found to be calorimetrically irreversible and scan-rate dependent. Calorimetric determinations of stability parameters of proteins which unfold irreversibly according to a first-order kinetic scheme have been reported. These methods require...read more

Mentioned in this Paper

Inorganic phosphate
Phosphate ion
Proteolytic Enzyme
Natto Bacteria
Regression Analysis

Related Papers

Paper Details
References
  • References20
  • Citations9
12

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