Stability, structural and suicide inactivation changes of mushroom tyrosinase after acetylation by N-acetylimidazole

International Journal of Biological Macromolecules
A A SabouryB Farzami

Abstract

Modification (acetylation) of Tyr residues with N-acetylimidazole protects outstandingly mushroom tyrosinase (MT) from the suicide inactivation in the presence of its catecholic substrate, 4-[(4-methylbenzo) azo]-1,2-benzenediol. UV spectrophotometric experiments and differential scanning calorimetry (DSC) studies indicated a decrease in kinetic stability of the enzyme alongside with increase in its thermal stability as well as its stability against n-dodecyl trimethylammonium bromide as a denaturizing agent. Pace analysis resulted in standard Gibbs free energy values of 46.54 and 52.09 kJ/mol in the absence of denaturant for native and modified enzyme, respectively. Structural studies by circular dichroism (CD) spectrophotometry showed that modification did not have major impact on the secondary structure of MT; however, induced some changes in its tertiary structure. The near-UV CD results revealed that the modification had enhanced intramolecular van der Waals interactions in the enzyme structure, which was in coincidence with its thermodynamic stability.

References

Jun 1, 1991·European Journal of Biochemistry·A J Winder, H Harris
Oct 1, 1988·Medicinal Research Reviews·G Prota
Apr 30, 1987·Biochimica Et Biophysica Acta·F García CánovasJ A Lozano
Jan 1, 1986·Methods in Enzymology·J T YangH M Martinez
Jan 1, 1983·The International Journal of Biochemistry·J D GalindoJ A Lozano
Jan 1, 1981·Critical Reviews in Food Science and Nutrition·L Vámos-Vigyázó
Feb 22, 1995·Biochimica Et Biophysica Acta·A Sánchez-FerrerF García-Carmona
Apr 13, 1994·Biochimica Et Biophysica Acta·M García MorenoF García Cánovas
Mar 11, 1996·FEBS Letters·E StratilováH Jörnvall
Apr 4, 1997·Biochimica Et Biophysica Acta·S M Kelly, N C Price
Aug 1, 1997·Phytochemistry·C W van GelderH J Wichers
Dec 23, 1999·Current Opinion in Structural Biology·C EickenJ C Sacchettini
Sep 21, 2000·European Journal of Biochemistry·L G FenollF García-Cánovas
Dec 6, 2002·The Biochemical Journal·Elmar Jaenicke, Heinz Decker
Jan 18, 2003·Journal of Enzyme Inhibition and Medicinal Chemistry·Antonio RescignoEnrico Sanjust
May 2, 2003·Journal of Agricultural and Food Chemistry·Sung-Yum SeoNiti Sharma
May 21, 2003·Accounts of Chemical Research·Edward J LandPatrick A Riley
May 23, 2003·Biotechnology and Applied Biochemistry·Neeru Munjal SharmaSuriender Kumar Sawhney
May 27, 2003·Biochemistry. Biokhimii︠a︡·Li-Ping XieRong-Qing Zhang
Jan 1, 1972·Methods in Enzymology·J F Riordan, B L Vallee
Jan 1, 1972·Methods in Enzymology·J F Riordan, B L Vallee
Jan 1, 1972·Methods in Enzymology·J F Riordan, B L Vallee

❮ Previous
Next ❯

Citations

Sep 29, 2006·Journal of Biosciences·N GheibiA A Moosavi-Movahedi
Feb 6, 2009·The Protein Journal·Neguine Rezaii, Fariba Khodagholi
Jun 13, 2012·Journal of Biomolecular Structure & Dynamics·Mahdi AlijanianzadehAli Akbar Moosavi-Movahedi
Nov 13, 2004·Biochimica Et Biophysica Acta·Kamahldin HaghbeenFarhad Karbassi
Jan 27, 2007·Journal of Enzyme Inhibition and Medicinal Chemistry·A A SabouryA A Moosavi-Movahedi
Oct 7, 2005·Journal of Enzyme Inhibition and Medicinal Chemistry·N GheibiA A Moosavi-Movahedi
May 24, 2007·Journal of Enzyme Inhibition and Medicinal Chemistry·M AlijanianzadehA A Moosavi-Movahedi
Feb 9, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Samaneh ZolghadriAli Akbar Saboury
Feb 13, 2021·Bioscience, Biotechnology, and Biochemistry·Takeharu MinoItaru Hamachi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
S Shareefi BorojerdiA A Saboury
Colloids and Surfaces. B, Biointerfaces
N GheibiA A Moosavi-Movahedi
Protein Science : a Publication of the Protein Society
Narasimha Sreerama, Robert W Woody
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Hong-Wei ZhouHai-Meng Zhou
© 2021 Meta ULC. All rights reserved