Stabilization by urea during thermal unfolding-mediated aggregation of recombinant human interleukin-1 receptor (type II): does solvation entropy play a role?

The Journal of Physical Chemistry. B
Richard L RemmeleLei Yu

Abstract

The protein denaturing properties of urea are well-known and still the subject of debate. It has been noted that in some cases where urea concentrations are relatively low stabilization is afforded against aggregation. An explanation for this unusual effect has seemingly remained elusive. Evidence is offered to propose urea stabilization is related to its influence on the solvation property of the protein molecules when in contact with an unfolded hydrophobic surface that tends to increase the entropy of the local aqueous solvent. This property of urea is expected to lower the entropic driving force of unfolded-mediated aggregation despite the increase in enthalpy. The data presented from toluene transfer experiments into 2 M urea + 0.1 M sodium phosphate solutions showed that the solvation free energy change was negative up to ∼75 °C. The associated ΔΔH was positive, leading to the conclusion that entropy drives the solvation process within the temperature domain from ∼20° to 75 °C. Using thermodynamic parameters from the toluene solvation experiments, it was possible to accurately determine the T(m) shift of recombinant human interleukin-1 receptor type II (rhuIL-1R(II)). Heating experiments above the apparent T(m) in the sam...Continue Reading

References

Jan 1, 1979·Advances in Protein Chemistry·P L Privalov
Jul 20, 1992·Journal of Molecular Biology·G I Makhatadze, P L Privalov
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R L Baldwin
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T OoiH A Scheraga
Jul 5, 1974·Journal of Molecular Biology·P L Privalov, N N Khechinashvili
Dec 7, 1982·Biochemistry·T Arakawa, S N Timasheff
Jan 1, 1995·Journal of Pharmaceutical Sciences·D B VolkinC R Middaugh
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S N Timasheff
Feb 1, 2002·Biochemical and Biophysical Research Communications·F EdwinM V Jagannadham
Feb 14, 2002·Journal of the American Chemical Society·Qin ZouKenneth P Murphy
Nov 6, 2002·Biochemistry·Abani K Bhuyan
Mar 26, 2003·Protein Expression and Purification·Kouhei TsumotoTsutomu Arakawa
Sep 23, 2003·Biophysical Chemistry·A K SoperAlenka Luzar
Oct 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·D K KlimovD Thirumalai
Aug 23, 2005·Biophysical Journal·Jörg RösgenDavid Wayne Bolen
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M M Teeter
Apr 13, 2006·Journal of the American Chemical Society·Maeng-Eun Lee, Nico F A van der Vegt
Oct 10, 2006·The AAPS Journal·Mary E M CromwellFred Jacobson
Dec 19, 2006·Journal of Biochemistry·Nuzhat GullRizwan Hasan Khan
Aug 21, 2007·Biotechnology and Bioengineering·Christopher J Roberts
Jun 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kalyan K Sinha, Jayant B Udgaonkar
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Oyvind HalskauJose M Sanchez-Ruiz
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peter J Rossky
Jan 7, 2009·Journal of the American Chemical Society·Ronen ZangiB J Berne
May 6, 2009·Biophysical Journal·Martin C Stumpe, Helmut Grubmüller
Jul 20, 2011·Physical Chemistry Chemical Physics : PCCP·Athi N NaganathanJose M Sanchez-Ruiz

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