Stabilization of a formate dehydrogenase by covalent immobilization on highly activated glyoxyl-agarose supports

Biomacromolecules
Juan M BolivarCesar Mateo

Abstract

Formate dehydrogenase (FDH) is a stable enzyme that may be readily inactivated by the interaction with hydrophobic interfaces (e.g., due to strong stirring). This may be avoided by immobilizing the enzyme on a porous support by any technique. Thus, even if the enzyme is going to be used in an ultra-membrane reactor, the immobilization presents some advantages. Immobilization on supports activated with bromocianogen, polyethylenimine, glutaraldehyde, etc., did not promote any stabilization of the enzyme under thermal inactivation. However, the immobilization of FDH on highly activated glyoxyl agarose has permitted increasing the enzyme stability against any distorting agent: pH, T, organic solvent, etc. The time of support-enzyme reaction, the temperature of immobilization, and the activation of the support need to be optimized to get the optimal stability-activity properties. Optimized biocatalyst retained 50% of the offered activity and became 50 times more stable at high temperature and neutral pH. Moreover, the quaternary structure of this dimeric enzyme becomes stabilized by immobilization under optimized conditions. Thus, at acidic pH (conditions where the subunit dissociation is the first step in the enzyme inactivation),...Continue Reading

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Citations

Jun 12, 2008·Applied Microbiology and Biotechnology·Juan M BolivarRoberto Fernandez-Lafuente
Feb 2, 2008·Bioprocess and Biosystems Engineering·L HilterhausA Liese
May 27, 2014·Biotechnology Advances·João Pinto da CostaRui Vitorino
Jun 29, 2013·Chemical Society Reviews·Andreas S Bommarius, Mariétou F Paye
Jan 25, 2012·Colloids and Surfaces. B, Biointerfaces·Joey N Talbert, Julie M Goddard
Aug 16, 2011·Journal of Biotechnology·Juan M BolivarFernando López-Gallego
Mar 19, 2014·Biopolymers·Sarah Nawel GasmiDidier Le Cerf
Jan 12, 2013·International Journal of Molecular Sciences·Raushan Kumar SinghJung-Kul Lee
Feb 13, 2018·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Cong ChaoBing Zhang
Sep 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jose J Virgen-OrtízRoberto Fernandez-Lafuente
Nov 25, 2016·Applied Biochemistry and Biotechnology·César Rafael Fanchini TerrasanJosé Manuel Guisan
Feb 24, 2021·International Journal of Biological Macromolecules·Giuseppe PietricolaMarco Piumetti
May 22, 2021·Applied Biochemistry and Biotechnology·Sabrina Ait BrahamRoberto Fernandez-Lafuente
Aug 30, 2021·Biotechnology Advances·Rafael C RodriguesRoberto Fernandez-Lafuente

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