Stabilization of a full-length infectious cDNA clone of transmissible gastroenteritis coronavirus by insertion of an intron

Journal of Virology
José M GonzálezLuis Enjuanes

Abstract

The stable propagation of a full-length transmissible gastroenteritis coronavirus (TGEV) cDNA in Escherichia coli cells as a bacterial artificial chromosome has been considerably improved by the insertion of an intron to disrupt a toxic region identified in the viral genome. The viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and the intron was efficiently removed during translocation of this RNA to the cytoplasm. The insertion in two different positions allowed stable plasmid amplification for at least 200 generations. Infectious TGEV was efficiently recovered from cells transfected with the modified cDNAs.

References

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Citations

Oct 28, 2004·Journal of Virology·Fernando AlmazánLuis Enjuanes
Mar 16, 2006·Journal of Virology·Julien R St-JeanPierre J Talbot
Jun 17, 2014·Virus Research·Fernando AlmazánLuis Enjuanes
Jul 24, 2010·Interdisciplinary Perspectives on Infectious Diseases·Jayasri Das Sarma
Feb 16, 2010·Virus Research·Leonor C Bedoya, José-Antonio Daròs
May 17, 2015·The Journal of General Virology·Juggragarn JengarnAnan Jongkaewwattana
Sep 19, 2006·Virology·Sonia ZúñigaLuis Enjuanes

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