Jun 1, 1976

Stabilization of a psychrotrophic Pseudomonas protease by calcium against thermal inactivation in milk at ultrahigh temperature

Applied and Environmental Microbiology
J T BarachM L Speck

Abstract

The heat-stable extracellular protease of Pseudomonas sp. (isolate MC60) was investigated. Heat resistance of the enzyme in milk at sterilization temperature was dependent on the presence of Ca2+. The half-life of the enzyme at ultrahigh temperature (149 C) in skim milk or milk-salts buffer with Ca2+ was approximately 7.0 s. Treatment of milk with chelators completely removed the heatstabilizing effect of milk. The enzyme was partially purified by ammonium sulfate precipitation and column chromatography on Sephadex G-100. At 21 C the enzyme retained greater than 85% activity after exposure to pH values between 5 and 10. Enzyme activity was reduced by metal chelating agents. Both Ca2+ and Zn2+ were required for optimal enzyme activity. Molecular weight was estimated at 48,000 by gel filtration.

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Mentioned in this Paper

Pseudomonas Infections
Calcium [EPC]
Extracellular
Calcium
Peptide Hydrolases
Food Preservation
Chelating Agents
Endopeptidases
Proteolytic Enzyme
Edetic Acid, Sodium Salt

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