Stabilization of Cu(I) for binding and calorimetric measurements in aqueous solution

Dalton Transactions : an International Journal of Inorganic Chemistry
Destinee K JohnsonNicholas E Grossoehme

Abstract

Conditions have been developed for the comproportionation reaction of Cu(2+) and copper metal to prepare aqueous solutions of Cu(+) that are stabilized from disproportionation by MeCN and other Cu(+)-stabilizing ligands. These solutions were then used in ITC measurements to quantify the thermodynamics of formation of a set of Cu(+) complexes (Cu(I)(MeCN)3(+), Cu(I)Me6Trien(+), Cu(I)(BCA)2(3-), Cu(I)(BCS)2(3-)), which have stabilities ranging over 15 orders of magnitude, for their use in binding and calorimetric measurements of Cu(+) interaction with proteins and other biological macromolecules. These complexes were then used to determine the stability and thermodynamics of formation of a 1 : 1 complex of Cu(+) with the biologically important tri-peptide glutathione, GSH. These results identify Me6Trien as an attractive Cu(+)-stabilizing ligand for calorimetric experiments, and suggest that caution should be used with MeCN to stabilize Cu(+) due to its potential for participating in unquantifiable ternary interactions.

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Citations

Sep 4, 2015·Biochimica Et Biophysica Acta·Rachel A JohnsonNicholas E Grossoehme
Jul 26, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Rupali G ShindeAtanu Barik
Oct 3, 2020·Scientific Reports·Søren BranderKatja S Johansen
Aug 9, 2020·Metallomics : Integrated Biometal Science·Daniel J Kosman
Apr 30, 2017·Chemical Science·Stefanie N GeigleDennis G Gillingham
Nov 5, 2017·The Journal of Biological Chemistry·M Thomas MorganChristoph J Fahrni
Jun 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Anastasia C ManesisAmy C Rosenzweig
Oct 25, 2017·Inorganic Chemistry·Sarah K GibbonsHoward H Patterson
Aug 23, 2019·Journal of the American Chemical Society·Molly L North, Dean E Wilcox

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