Stabilization of dentin matrix after cross-linking treatments, in vitro

Dental Materials : Official Publication of the Academy of Dental Materials
Débora L S ScheffelDavid H Pashley

Abstract

To evaluate the effect of EDC on elastic modulus (E), MMPs activity, hydroxyproline (HYP) release and thermal denaturation temperature of demineralized dentin collagen. Dentin beams were obtained from human molars and completely demineralized in 10 wt% H3PO4 for 18 h. The initial E and MMP activity were determined with three-point bending and microcolorimetric assay, respectively. Extra demineralized beams were dehydrated and the initial dry mass (DM) was determined. All the beams were distributed into groups (n=10) and treated for 30 s or 60 s with: water, 0.5 M, 1 M or 2 M EDC or 10% glutaraldehyde (GA). After treatment, the new E and MMP activity were redetermined. The beams submitted to DM measurements were storage for 1 week in artificial saliva, after that the mass loss and HYP release were evaluated. The collagen thermal denaturation temperature (TDT) was determined by DSC analysis. Data for E, MMP activity and HYP release were submitted to Wilcoxon and Kruskal-Wallis or Mann-Whitney tests. Mass loss and TDT data were submitted to ANOVA and Tukey tests at the 5% of significance. EDC was able to significantly increase collagen stiffness in 60s. 10% GA groups obtained the highest E values after both 30 and 60s. All cross-l...Continue Reading

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Citations

May 23, 2014·Dental Materials : Official Publication of the Academy of Dental Materials·Camila SabatiniDavid H Pashley
Jan 9, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Gianluca TurcoLorenzo Breschi
Jan 9, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Andrea FrassettoMilena Cadenaro
Jan 18, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Viviane HassAlessandra Reis
Dec 31, 2015·European Journal of Oral Sciences·Masanori HashimotoSatoshi Imazato
Jan 15, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Milena CadenaroLorenzo Breschi
Oct 30, 2015·European Journal of Oral Sciences·Guus van StrijpYouichi Iijima
Apr 19, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Viviane HassAlessandra Reis
May 20, 2015·Journal of Dentistry·Débora Lopes Salles ScheffelJosimeri Hebling
Jul 20, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Jianfeng ZhouDavid H Pashley
Jan 23, 2016·Operative Dentistry·F ShafieiM Mohammadi-Bassir
Jun 5, 2015·Journal of Dental Research·M HashimotoS Imazato
May 13, 2017·Journal of Dental Research·A MazzoniL Breschi
Feb 23, 2020·International Journal of Paediatric Dentistry·Debora Lopes Salles ScheffelJosimeri Hebling
Nov 20, 2016·European Journal of Oral Sciences·Joshua Lee, Camila Sabatini
Oct 24, 2017·Calcified Tissue International·J CaiM F Burrow
Aug 26, 2020·Restorative Dentistry & Endodontics·Izadora Quintela Souza de MoraesIsabel Cristina Celerino de Moraes Porto
Mar 2, 2021·European Journal of Oral Sciences·Diletta ForgioneArzu Tezvergil-Mutluay
Mar 6, 2021·Heliyon·Isabel Cristina Celerino de Moraes PortoJohnnatan Duarte de Freitas
Jun 12, 2020·Journal of Dentistry·Kang LiCynthia Kar Yung Yiu
Nov 29, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Annalisa MazzoniLorenzo Breschi
Oct 9, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·C Parise GréBart Van Meerbeek
Sep 9, 2019·ACS Biomaterials Science & Engineering·Leping WuQuanli Li

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