Stabilization of Enzymes against Thermal Stress and Freeze-Drying by Mannosylglycerate.

Applied and Environmental Microbiology
Ana RamosHelena Santos

Abstract

2-O-(beta)-Mannosylglycerate, a solute that accumulates in some (hyper)thermophilic organisms, was purified from Pyrococcus furiosus cells, and its effect on enzyme stabilization in vitro was assessed. Enzymes from hyperthermophilic, thermophilic, and mesophilic sources were examined. The thermostabilities of alcohol dehydrogenases from P. furiosus and Bacillus stearothermophilus and of glutamate dehydrogenases from Thermotoga maritima and Clostridium difficile were improved to a significant extent when enzyme solutions were incubated at supraoptimal temperatures in the presence of 2-O-(beta)-mannosylglycerate, but no effect on the thermostability of glutamate dehydrogenase from P. furiosus was detected. On the other hand, there was a remarkable effect on the thermal stabilities of rabbit muscle lactate dehydrogenase, baker's yeast alcohol dehydrogenase, and bovine liver glutamate dehydrogenase, which were used as model systems to evaluate stabilization of enzymes of mesophilic origin. For all of the enzymes examined and at the highest temperatures tested, 2-O-(beta)-mannosylglycerate was a better thermoprotectant than trehalose. The stabilizing effect exerted by 2-O-(beta)-mannosylglycerate on enzymes suggests a role for this ...Continue Reading

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Citations

Dec 1, 2009·Biotechnology Journal·Thornthan SawangwanBernd Nidetzky
Sep 8, 2006·Applied Microbiology and Biotechnology·Georg Lentzen, Thomas Schwarz
Jan 4, 2015·Extremophiles : Life Under Extreme Conditions·Pedro LamosaHelena Santos
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Sep 11, 2002·Environmental Microbiology·Helena Santos, Milton S da Costa
Nov 20, 2019·Journal of Food Science and Technology·Hilal IslerogluMehmet Tokatli
Jun 11, 2005·The Journal of Chemical Physics·Sau Lawrence LeeJeffrey R Errington
Aug 3, 2005·Extremophiles : Life Under Extreme Conditions·Snaedis H BjornsdottirJakob K Kristjansson
Mar 11, 2011·Natural Product Reports·Christiane Luley-Goedl, Bernd Nidetzky
Mar 12, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Susana GonçalvesPedro M Matias
Apr 22, 2011·Extremophiles : Life Under Extreme Conditions·Pedro LamosaGarabed Antranikian
May 24, 2007·PloS One·Y-H Percival ZhangMichael W W Adams
Feb 16, 2002·Environmental Microbiology·M Roesser, V Müller
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