Jul 1, 1976

Stabilization of human serum alkaline phosphatase to histidine-induced heat inactivation by tryptic digestion

Journal of Biochemistry
T YamashitaM Mori

Abstract

1. Serum alkaline phosphatase [EC 3.1.3.1] was strongly inactivated by histidine during incubation at pH 8.0 and 45degrees; however, tryptic digestion of the serum strongly protected the enzyme against inactivation by histidine. In the absence of histidine, however, neither heat inactivation of the phosphatase nor the effect of trypsin [EC 3.4.21.4] was observed. Factors affecting the alkaline phosphatase inactivation were studied further. 2. The effect of trypsin on the histidine-induced heat inactivation differed considerably according to the tissue source of the enzyme, which suggests a possible method for distinguishing alkaline phosphatase isoenzymes.

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Mentioned in this Paper

Phosphoric Monoester Hydrolases
Histidine
Cathepsin G
Alkaline Phosphatase Measurement
Hot Temperature
Alkaline phosphatase isoenzyme
Placenta
Alkaline Phosphatase
Trypsin
Edetic Acid, Sodium Salt

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