Stabilization of Immobilized Enzymes via the Chaperone-Like Activity of Mixed Lipid Bilayers

ACS Applied Materials & Interfaces
Andres F Chaparro SosaDaniel K Schwartz

Abstract

Biomimetic lipid bilayers represent intriguing materials for enzyme immobilization, which is critical for many biotechnological applications. Here, through the creation of mixed lipid bilayers, the retention of immobilized enzyme structures and catalytic activity are dramatically enhanced. The enhancement in the retention of enzyme structures, which correlated with an increase in enzyme activity, is observed using dynamic single-molecule (SM) fluorescence methods. The results of SM analysis specifically show that lipid bilayers composed of mixtures of 1,2-dioleoyl- sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl- sn-glycero-3-phospho-(1'- rac-glycerol) (DOPG) stabilize the folded state of nitroreductase (NfsB), increasing the rate of refolding relative to unfolding of enzyme molecules on the bilayer surface. Remarkably, for optimal compositions with 15-50% DOPG, over 95% of NfsB remains folded while the activity of the enzyme is increased as much as 2 times over that in solution. Within this range of DOPG, the strength of the interaction of folded and unfolded NfsB with the bilayer surface was also significantly altered, which was evident by the change in the diffusion of folded and unfolded NfsB in the bilayer. Ultimately,...Continue Reading

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Citations

Apr 2, 2020·International Journal of Molecular Sciences·Manuel TorresPablo Vicente Escribá
Feb 3, 2021·Biophysical Chemistry·Fulvio Grigolato, Paolo Arosio
Mar 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Connor J ThompsonDaniel K Schwartz
Apr 30, 2021·Journal of the American Chemical Society·Andres F Chaparro SosaJoel L Kaar
Aug 2, 2018·ACS Nano·Daniel F KienleDaniel K Schwartz
Nov 3, 2020·The Journal of Physical Chemistry Letters·Marcelo H GehlenDaniel K Schwartz
May 1, 2020·ACS Applied Materials & Interfaces·Daniel F KienleDaniel K Schwartz
Aug 18, 2020·The Journal of Physical Chemistry Letters·Andres F Chaparro SosaJoel L Kaar
Jan 28, 2020·Journal of the American Chemical Society·James S WeltzJoel L Kaar
May 9, 2019·The Journal of Physical Chemistry Letters·David S BullAndrew P Goodwin
Oct 1, 2021·Journal of the American Chemical Society·Albert Velasco AbadiaJoel L Kaar

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