Stabilization of indocyanine green dye in polymeric micelles for NIR-II fluorescence imaging and cancer treatment

Biomaterials Science
Gil YeroslavskyKohei Soga

Abstract

One of the most commonly used near infrared (NIR) dyes is indocyanine green (ICG), which has been extensively used for NIR bioimaging, photothermal and photodynamic therapy. However, upon excitation this dye can react with molecular oxygen to form singlet oxygen (SO), which can then cleave ICG to form non-fluorescent debris. In order to reduce the reaction between ICG and oxygen, we used energy transfer (ET) between the former and the NIR dye IR-1061. The two dyes were encapsulated in micelles composed of biocompatible poly(ethylene glycol)-block-poly(ε-caprolactone) (PCL-PEG). Micelles were characterized for their size using dynamic light scattering (DLS) and were found to measure about 35 nm in diameter. Fluorescence emission measurements were conducted to show that the stability of ICG against photodecomposition is increased. Moreover, this increased stability allows the encapsulated dye to generate more heat and for a longer time, compared to its free form. Studies with a SO indicator showed that as more IR-1061 is added to the micelles, less SO is produced. These results show how by changing the amount of added IR-1061 it is possible to tune the heat and SO generated by the system. Cell viability studies demonstrated that ...Continue Reading

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Citations

May 11, 2020·Journal of Biophotonics·Masakazu UmezawaKohei Soga
Jan 29, 2021·Biomedicines·Su Woong YooEuiheon Chung
Dec 22, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Masakazu UmezawaKohei Soga
Jul 3, 2021·International Journal of Molecular Sciences·Giulia Della PelleNina Kostevšek

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Methods Mentioned

BETA
FRET
dynamic light scattering
light scattering

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