Stabilization of plakoglobin and enhanced keratinocyte cell-cell adhesion by intracellular O-glycosylation

The Journal of Biological Chemistry
Peiqi HuDavid S Rubenstein

Abstract

O-Glycosylation modifies and regulates a variety of intracellular proteins. Plakoglobin, which functions in both cell-cell adhesion and signal transduction, is modified by O-glycosylation; however, the significance is unknown. To investigate the functional consequence of plakoglobin O-glycosylation, we cloned and overexpressed in keratinocytes murine O-GlcNAc transferase (mOGT). Over expression of mOGT in murine keratinocytes resulted in (i) glycosylation of plakoglobin and (ii) increased levels of plakoglobin due to post-translational stabilization of plakoglobin. Additionally, overexpression of mOGT in keratinocytes correlated with increased staining for cell-cell adhesion proteins and greater cell-cell adhesion. These observations suggest that O-glycosylation functions to regulate the post-translational stability of plakoglobin and keratinocyte cell-cell adhesion.

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Citations

May 22, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jiřina ProcházkováJan Vondráček
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Jan 9, 2014·The Journal of Biological Chemistry·Kasper RunagerDavid S Rubenstein
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Jul 1, 2011·Journal of Biomedical Research·Meryem Bektas, David S Rubenstein

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