Abstract
Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 72 amino acids of PtlD (amino acids 392 to 463) was sufficient for maintaining the stability of PtlE, PtlF, and PtlH, although this region was not sufficient to support secretion of the toxin. Further analysis demonstrated that a stretch of 10 amino acids at the C-terminal end of PtlD (amino acids 425 to 434) contributes to transporter stability.
References
Jul 1, 1991·Journal of Bacteriology·S Stibitz, M S Yang
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A NicosiaR Rappuoli
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1981·Analytical Biochemistry·W N Burnette
Jan 1, 1984·Infection and Immunity·A A Weiss, S Falkow
Jan 1, 1994·Methods in Enzymology·S Stibitz
Sep 1, 1994·Journal of Bacteriology·F D Johnson, D L Burns
Mar 1, 1993·Molecular Plant-microbe Interactions : MPMI·R De FeyterD W Gabriel
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A A WeissD L Burns
Jan 1, 1996·Methods in Enzymology·J GarnierB Robson
Feb 1, 1996·Trends in Microbiology·S C WinansP J Christie
Dec 6, 1996·The Journal of Biological Chemistry·K M FarizoD L Burns
Sep 24, 1999·Journal of Molecular Biology·D T Jones
Oct 16, 1999·FEMS Microbiology Letters·K A Craig-Mylius, A A Weiss
Mar 1, 2000·Trends in Biochemical Sciences·C CombetG Deléage
Jun 17, 2000·Infection and Immunity·K M FarizoD L Burns
Jul 27, 2000·Journal of Bacteriology·S HapfelmeierC Baron
Apr 18, 2003·Journal of Bacteriology·Simon J JakubowskiPeter J Christie
Mar 25, 2004·Infection and Immunity·Anissa M CheungDrusilla L Burns
Aug 27, 2004·Journal of Molecular Biology·Simon J JakubowskiPeter J Christie
Dec 23, 2004·Molecular Microbiology·Paul K JuddAnath Das
Jun 28, 2005·Nucleic Acids Research·Kevin BrysonDavid T Jones
Sep 13, 2005·Annual Review of Microbiology·Peter J ChristieEric Cascales
Mar 7, 2007·Infection and Immunity·Anita Verma, Drusilla L Burns
Citations
Jan 10, 2013·PloS One·Tuan M NguyenTzvia Abramson
Mar 10, 2010·Future Microbiology·Nicholas H Carbonetti
Aug 30, 2014·FEMS Microbiology Reviews·Joseph J GillespieAbdu F Azad
Jul 12, 2011·The FEBS Journal·Camille LochtNathalie Mielcarek
Jan 6, 2011·FEMS Microbiology Reviews·Daan de GouwFrits R Mooi