Stabilizing and destabilizing effects of arginine on deoxyribonucleic acid

International Journal of Biological Macromolecules
T ArakawaA Hajime Koyama

Abstract

Aqueous arginine solution now finds a wide range of applications in biotechnology fields, including protein refolding, chromatography and virus inactivation. While progress has been made for mechanistic understanding of the effects of arginine on proteins, we have little understanding on how arginine inactivates viruses. One of the viral components is nucleic acid. We have examined the effects of arginine on the structure and thermal stability of calf thymus deoxyribonucleic acid (DNA) using circular dichroism (CD). Both NaCl and arginine reduced CD intensity. At low concentrations, arginine showed a stronger effect on CD intensity than NaCl. Both NaCl and arginine sharply increased the melting temperature at low concentrations (below 0.25 M). However, they had an opposite effect at higher concentrations. Above this concentration, NaCl gradually increased the melting temperature, leading to the onset melting temperature above 90 degrees C. On the other hand, the thermal stability in the presence of arginine reached a maximum at 0.2-0.5 M, after which further addition of arginine caused decreased melting temperature. It is most likely that the increased melting temperature at low concentration is due to electrostatic stabilizati...Continue Reading

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Citations

May 17, 2011·The Journal of Physical Chemistry. B·Curtiss P SchneiderBernhardt L Trout
Jun 4, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tsutomu Arakawa
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Mar 30, 2010·Archives of Biochemistry and Biophysics·Atsushi HiranoKentaro Shiraki

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