Stable carbon isotope fractionation in chlorinated ethene degradation by bacteria expressing three toluene oxygenases.

Frontiers in Microbiology
Scott C ClingenpeelMary E Watwood

Abstract

One difficulty in using bioremediation at a contaminated site is demonstrating that biodegradation is actually occurring in situ. The stable isotope composition of contaminants may help with this, since they can serve as an indicator of biological activity. To use this approach it is necessary to establish how a particular biodegradation pathway affects the isotopic composition of a contaminant. This study examined bacterial strains expressing three aerobic enzymes for their effect on the (13)C/(12)C ratio when degrading both trichloroethene (TCE) and cis-1,2-dichloroethene (c-DCE): toluene 3-monoxygenase, toluene 4-monooxygenase, and toluene 2,3-dioxygenase. We found no significant differences in fractionation among the three enzymes for either compound. Aerobic degradation of c-DCE occurred with low fractionation producing δ(13)C enrichment factors of -0.9 ± 0.5 to -1.2 ± 0.5, in contrast to reported anaerobic degradation δ(13)C enrichment factors of -14.1 to -20.4‰. Aerobic degradation of TCE resulted in δ(13)C enrichment factors of -11.6 ± 4.1 to -14.7 ± 3.0‰ which overlap reported δ(13)C enrichment factors for anaerobic TCE degradation of -2.5 to -13.8‰. The data from this study suggest that stable isotopes could serve as ...Continue Reading

Citations

Oct 13, 2012·Critical Reviews in Biotechnology·Awadhesh Kumar ShuklaSuresh Kumar Dubey
May 24, 2016·The Journal of General and Applied Microbiology·Kenta YonezukaMasao Fukuda
Dec 6, 2018·Environmental Science & Technology·Benno N EhrlMartin Elsner

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