PMID: 8614281Jan 1, 1996Paper

Stable expression and heterologous coupling of the kappa opioid receptor in cell lines of neural and nonneural origin

Life Sciences
P BanerjeeG Dawson

Abstract

Signal transduction cascades initiated by the neuronal kappa opioid receptor were studied following transfection of a neuronal (hippocampal) line, HN2, and the non-neural CHOs. Retinoic-acid mediated differentiation resulted in intense staining of the HN2 cells with a neurofilament protein antibody SMI 33 but not with an antibody to GFAP, thus establishing neuronal characteristics of the HN2 cell line. The kappa opioid receptor was stably expressed in the two cell lines by electroporation mediated transfer of a Cytomegalovirus-promoter driven construct, pCMV-kappa, harboring the kappa-opioid receptor cDNA. Positive clones (HN2 kappa 24 and CHO kappa 18) from both lines showed high expression of the kappa opioid receptor, as identified by [3H] U-69,593 binding to membranes prepared from HN2 kappa 24 and CHO kappa 18. Scatchard analysis revealed the presence of high affinity kappa opioid receptors in both engineered cell lines (KD=1.3 nM for HN2 kappa 24 and 2.1 nM for CHO kappa 18). Functional coupling to adenylate cyclase was displayed by 1 microM U-69,593 mediated inhibition (55-63%) of prostaglandin E1-stimulated intracellular cAMP levels. A major difference between the two clones was observed in functional coupling of the ex...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1990·Life Sciences·S Periyasamy, W Hoss
May 7, 1990·Biochimica Et Biophysica Acta·L BirnbaumerA M Brown
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D PlatikaM C Fishman
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·M NirenbergH Fukui
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·B C SwarzenskiR D Todd
Feb 15, 1993·Journal of Neuroscience Research·G DawsonG R Post
Apr 15, 1993·Biochemical and Biophysical Research Communications·P BanerjeeG Dawson

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Citations

Nov 25, 2005·Bioscience Reports·Tatyana AdayevProbal Banerjee
Sep 25, 2007·Chemistry and Physics of Lipids·Md Jafurulla, Amitabha Chattopadhyay
Apr 23, 2008·Current Protocols in Neuroscience·H Potter

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