Stable expression in HEK-293 cells of the rat alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor

FEBS Letters
E StetzerA Maelicke

Abstract

The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by single-cell and whole-cell patch-clamp studies. Acetylcholine (ACh) induced fast activating currents which showed desensitisation and inward rectification. The conductance of the ACh-activated channel was 29 pS. The order of potency of the nicotinic agonists tested was cytisine approximately = nicotine > acetylcholine. The EC50 value for ACh was 145 microM; the Hill coefficient was close to 2. The currents elicited by ACh were effectively blocked by nicotinic antagonists, but not by the muscarinic antagonist atropine. These properties are comparable to the pharmacological and physiological profile of ganglionic nicotinic receptors and type III currents of cultured hippocampal neurons.

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Citations

May 3, 2005·Journal of Pharmacological and Toxicological Methods·Philip Thomas, Trevor G Smart
Nov 19, 1997·Progress in Neurobiology·C GottiF Clementi
Mar 23, 2001·Acta Neurologica Scandinavica. Supplementum·M SamochockiA Maelicke
Mar 11, 2004·The European Journal of Neuroscience·Régis GrailhePierre-Jean Corringer
Jul 11, 1998·The Journal of Biological Chemistry·B HermsenA Maelicke
Mar 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·Marek SamochockiAlfred Maelicke
Apr 30, 2019·Frontiers in Neural Circuits·Cristina ColangeloSrikanth Ramaswamy

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