Stable expression of bioactive recombinant pleurocidin in a fish cell line

Applied Microbiology and Biotechnology
I BrocalA Estepa

Abstract

Pleurocidin (Ple), a linear cationic peptide of 25 amino acids, is a member of a larger family of antimicrobial peptides present in flatfish. Previous studies have shown that Ple displays a strong antimicrobial activity against a broad spectrum of bacteria and appears to play a role in innate host defence. In this work, the genomic sequence encoding the Ple prepropeptide has been isolated from Limanda limanda and cloned in a vector under the control of a non-viral promoter (the carp beta-actin promoter). By using this construction, expression of bioactive Ple was demonstrated in transformed fish cell lines continuously growing for more than 2 years. Furthermore, the study of Ple processing, maturation and secretion (by using fusion with green fluorescence protein) and the high bactericidal activity of the secreted recombinant Ple (detectable in cell supernatants without any concentration) are all reported here, as no other recombinant Ple or fish antimicrobial peptide have been expressed before to that extent. Such an overexpression of recombinant Ple or any other related antimicrobial peptide might improve the chances to develop new antibiotic agents, as well as to provide essential information about the mechanism of action, r...Continue Reading

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Citations

Mar 7, 2014·Pharmaceuticals·Jorge A Masso-Silva, Gill Diamond
Feb 15, 2013·The Journal of Investigative Dermatology·Sebastian RakersRalf Paus
Jan 22, 2017·Developmental and Comparative Immunology·Bertrand ColletKatherine Lester
Oct 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura Poisa-BeiroBeatriz Novoa

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