PMID: 2108694Jan 1, 1990Paper

Stable expression of human cytochrome P450IA2 cDNA in a human lymphoblastoid cell line: role of the enzyme in the metabolic activation of aflatoxin B1

Molecular Carcinogenesis
C L CrespiF J Gonzalez

Abstract

A human lymphoblastoid cell line stably expressing a human cytochrome P450IA2 cDNA was developed. This recombinant cell line displayed P450IA2 protein and estradiol 2-hydroxylase activity, neither of which was detected in the parental cell line. The recombinant cell line was also approximately 1000-fold more sensitive to the cytotoxicity and mutagenicity of the carcinogenic mycotoxin aflatoxin B1 than was the parent cell line. The increase in mutagenicity was supported by a corresponding increase in the level of aflatoxin B1 binding to DNA in cells expressing P450IA2 relative to control cells.

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Citations

Oct 26, 1995·Biochemical Pharmacology·D R GermolecM I Luster
Aug 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T YabeY Ueno
Nov 1, 1994·European Journal of Pharmacology·P PelkonenR O Juvonen
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Dec 2, 2004·Mutation Research·Guosheng Chen, Paul A White
Apr 1, 1992·Circulation·D BernsteinD Baum

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