Stable expression of insect GABA receptors in insect cell lines. Promoters for efficient expression of Drosophila and mosquito Rdl GABA receptors in stably transformed mosquito cell lines

FEBS Letters
F ShotkoskiR H ffrench-Constant

Abstract

We are interested in establishing stably transformed insect cell lines efficiently expressing the insect gamma-aminobutyric acid (GABA) receptor subunit gene Resistance to dieldrin or Rdl. In order to facilitate this we utilized a system based on stable transformation of Aedes albopictus mosquito cell lines using the dihydrofolate reductase (dhfr) gene as a selected marker. Here we report the production of stable mosquito cell lines carrying high copy numbers of Rdl genes from both Drosophila and Aedes aegypti mosquitoes and the subsequent high efficiency expression of functional GABA gated chlorine ion channels. We also used this system to compare the activity of a range of immediate early baculovirus promoters in mosquito cell culture and demonstrate that IE1 promoter constructs work efficiently across insect species. Results are discussed in relation to the potential use of these constructs in the generic transformation of non-Drosophilid insects.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T J MonroeB J Beaty
Jul 31, 1990·European Journal of Pharmacology·S J MossE A Barnard
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P P Di Nocera, I B Dawid
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M C WaltersD I Martin
Apr 30, 1993·Neuroscience Letters·B J HamiltonD B Carter
Dec 1, 1993·Insect Biochemistry and Molecular Biology·F A Shotkoski, A M Fallon
Nov 29, 1993·FEBS Letters·K A JoyceL A King
Jun 3, 1993·Nature·R H Ffrench-ConstantA E Chalmers
Jan 1, 1995·Journal of Receptor and Signal Transduction Research·L A SmithI Bermudez

❮ Previous
Next ❯

Citations

Nov 14, 1997·Current Opinion in Biotechnology·L McCarroll, L A King
Feb 2, 1999·Insect Molecular Biology·Y G Zhao, P Eggleston
Apr 13, 2000·Annual Review of Entomology·R H Ffrench-ConstantG Stilwell
Jul 14, 1998·Insect Molecular Biology·M AshburnerJ J Peloquin

❮ Previous
Next ❯

Related Concepts