Stable implantation of orthogonal sensor circuits in Gram-negative bacteria for environmental release

Environmental Microbiology
Aitor de Las HerasVíctor de Lorenzo

Abstract

A broad host range, orthogonal genetic platform has been developed to format sensor circuits in the chromosome of Gram-negative microorganisms destined for environmental release as bioindicators of toxic or perilous compounds (e.g. explosives) in soil. The genetic scheme includes the generation of a genomic landing pad for the sensor module with a Tn5-mini-transposon bearing an optimal attTn7 sequence and a choice of reporter systems with optical and enzymatic outputs. The array of functional elements thereby inserted in the chromosome match that of a cognate plasmid vector which delivers the transcription factors and the promoters to a frame that places the regulatory parts in front of the reporters. Site-specific recombination sites allow the deletion of antibiotic resistances and enables reporter output prior to deliberate release. The system thus allows the production and maintenance of cells in a pre-release state and its intentional conversion in deliverable strains that fulfil all safety, stability and performance criteria. The combination of such a genetic platform with a variant of the transcriptional regulator XylR of Pseudomonas putida that responds to 2,4-dinitrotoluene has been the basis for the production of strai...Continue Reading

References

Sep 1, 1994·Trends in Biotechnology·V de Lorenzo
Mar 21, 1998·Applied and Environmental Microbiology·B M WillardsonP J Jackson
May 14, 1999·Trends in Biotechnology·K N Timmis, D H Pieper
May 2, 2000·Applied and Environmental Microbiology·S F NishinoJ C Spain
Jun 14, 2000·Current Opinion in Biotechnology·G S Sayler, S Ripp
Oct 12, 2000·Current Opinion in Biotechnology·E Díaz, M A Prieto
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·J E Peters, N L Craig
Jul 11, 2002·Journal of Bacteriology·Glenn R JohnsonJim C Spain
May 9, 2003·Nature·Loren L LoogerHomme W Hellinga
Jul 2, 2003·Current Opinion in Microbiology·Shimshon Belkin
Apr 23, 2004·Current Opinion in Structural Biology·Mindy Steiniger-WhiteWilliam S Reznikoff
Sep 4, 2004·Environmental Microbiology·Jan Roelof van der MeerMarco Jaspers
Nov 25, 2004·Environmental Microbiology·V A P Martins Dos SantosK N Timmis
May 24, 2005·Nature Methods·Kyoung-Hee ChoiHerbert P Schweizer
Nov 25, 2005·Nature·Drew Endy
Dec 22, 2005·Current Opinion in Biotechnology·Joakim BjerketorpJanet K Jansson
Sep 7, 2006·Bioinformatics·Matthias Heinemann, Sven Panke
May 9, 2007·Nature Chemical Biology·Venkat RadhikaDanny N Dhanasekaran
May 29, 2007·Current Opinion in Biotechnology·Eliora Z Ron
Jul 25, 2007·Molecular Microbiology·Teca Calcagno GalvãoVíctor de Lorenzo
Jul 31, 2007·Biosensors & Bioelectronics·Maki K Habib
Feb 16, 2008·FEBS Letters·Rafael Silva-Rocha, Víctor de Lorenzo

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Citations

Nov 12, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Markus Schmidt, Lei Pei
May 23, 2012·Journal of Bacteriology·Macarena MarínDietmar H Pieper
Jan 12, 2010·Journal of Bacteriology·Macarena MarínDietmar H Pieper
Apr 26, 2013·Applied Microbiology and Biotechnology·Sharon Yagur-KrollShimshon Belkin
Jan 10, 2012·FEBS Letters·Antoine Danchin
Oct 28, 2011·Biotechnology Journal·Joseph A GredellPatrick C Cirino
Mar 21, 2012·Environmental Microbiology·Audrey LeprinceVitor A P Martins dos Santos
Mar 11, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Markus Schmidt
Jul 14, 2010·FEMS Microbiology Reviews·Aitor de Las HerasVíctor de Lorenzo
Nov 27, 2010·The International Journal of Biochemistry & Cell Biology·Mario Andrea Marchisio, Fabian Rudolf
Apr 17, 2014·Nature Reviews. Microbiology·Pablo I NikelVíctor de Lorenzo
Jun 2, 2010·Nature Reviews. Microbiology·Jan Roelof van der Meer, Shimshon Belkin
Jun 28, 2019·Frontiers in Bioengineering and Biotechnology·Christoph LenzenNick Wierckx
Apr 7, 2021·Nature Chemical Biology·Tzu-Chieh TangTimothy K Lu
May 10, 2021·Metabolic Engineering·Yannic S AckermannNick Wierckx
Jun 25, 2015·Microbiology Spectrum·Esteban Martínez-GarcíaVíctor De Lorenzo

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