PMID: 2107753Mar 1, 1990Paper

Stable incorporation of a bacterial gene into adult rat skeletal muscle in vivo

The American Journal of Physiology
D B Thomason, F W Booth

Abstract

We have developed a novel technique to incorporate and stably express foreign genes in adult rat skeletal muscle in vivo. Endogeneous satellite cells in skeletal muscle regenerating from bupivacaine damage were infected with an injected retrovirus containing the Escherichia coli beta-galactosidase gene under the promoter control of the Moloney murine leukemia virus long-terminal repeat. Constitutive and stable expression of beta-galactosidase activity was observed in muscle fibers after 6 days and 1 mo of muscle regeneration. Two patterns of expression were observed, diffuse expression within fibers and focal expression associated with the sarcolemma. This technique will allow future experiments with muscle-specific genes and promoters to study the physiological regulation of skeletal muscle gene expression in the intact adult mammal. Furthermore, the technique of stimulating stem cell proliferation to allow retroviral-mediated gene transfer may be generally applicable to other tissues.

References

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Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko

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Citations

Sep 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J E Morgan, T A Partridge
Oct 1, 1993·Chronobiology International·R B SothernS A Gruber
Nov 9, 2005·American Journal of Veterinary Research·Anna TidholmKerstin Hansson
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Priya Sethu ChockalingamDonald B Thomason
Jul 17, 1999·The American Journal of Physiology·D FreyssenetD A Hood
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·K W LiangL Huang
Jun 19, 1998·Neuromuscular Disorders : NMD·J C van DeutekomJ Huard
Jul 13, 2004·Vaccine·Huali JinBin Wang

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