Stable integration and expression of a bacterial gene in the mosquito Anopheles gambiae

Science
L H MillerH G Coon

Abstract

Foreign DNA was successfully introduced into the germline of the African mosquito vector of malaria Anopheles gambiae. Stable integration of genes into the germlines of insects had been achieved previously only in Drosophila melanogaster and related species and required the use of the P element transposon. In these experiments with Anopheles gambiae, the plasmid pUChsneo was used, which contains the selectable marker neo gene flanked by P element inverted repeats. Mosquitoes injected with this plasmid were screened for resistance to the neomycin analog G-418. A single event of plasmid insertion was recovered. Integration appears to be stable and, thus far, resistance to G-418 has been expressed for eight generations. The transformation event appears to be independent of P.

References

Jan 1, 1993·Archives of Insect Biochemistry and Physiology·A M HandlerD A O'brochta
Dec 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A Kimbrell
Jan 1, 1993·Molecular & General Genetics : MGG·A I NikolaevV Z Tarantul
Jul 8, 1994·Molecular & General Genetics : MGG·D A O'brochtaP W Atkinson
May 23, 2012·Molecular Genetics and Genomics : MGG·Elgion L S LoretoLizandra J Robe
Jul 20, 2006·Genetica·D A O'brochtaDerek J Charlwood
Feb 1, 1990·Parasitology Today·J M CramptonP Eggleston
Jun 1, 1992·Parasitology Today·N J Besansky, F H Collins
Apr 1, 1992·Parasitology Today·M Coluzzi
Dec 1, 1993·Parasitology Today·S HiggsK E Olson
Jan 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G D Baldridge, A M Fallon
Dec 1, 1993·Insect Biochemistry and Molecular Biology·F Shotkoski, A M Fallon
Nov 19, 2002·International Journal for Parasitology·L A MoreiraM Jacobs-Lorena
Nov 14, 1997·Current Opinion in Biotechnology·L McCarroll, L A King
Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Carsten HornErnst A Wimmer
Sep 13, 2002·Insect Biochemistry and Molecular Biology·A K GhoshM Jacobs-Lorena
Sep 1, 1996·Insect Biochemistry and Molecular Biology·D A O'brochta, P W Atkinson
May 1, 1997·Insect Biochemistry and Molecular Biology·A SarkarD A O'brochta
Aug 1, 1995·Nature Medicine·D M Wesson, D J Krogstad
Feb 2, 1999·Insect Molecular Biology·Y G Zhao, P Eggleston
Feb 15, 2000·Insect Molecular Biology·A C PinkertonP W Atkinson
Mar 10, 2001·Insect Molecular Biology·M F WalterH Biessmann
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D A O'brochta, A M Handler
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T J MonroeB J Beaty
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J K Presnail, M A Hoy
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Xiong, M Jacobs-Lorena
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T MatsubaraJ C Burns
Nov 11, 1991·Nucleic Acids Research·A C MorrisA A James
Jan 1, 1992·Insect Molecular Biology·M J KovachB J Beaty
Jan 1, 1993·Insect Molecular Biology·C B BeardS Aksoy
Jan 1, 1993·Insect Molecular Biology·M Q BenedictJ A Seawright
Nov 1, 1994·Insect Molecular Biology·F ShotkoskiR H ffrench-Constant
May 1, 1995·Insect Molecular Biology·A Blanchetot, R H Gooding
Nov 1, 1995·Insect Molecular Biology·A Rayms-KellerB J Beaty

Related Concepts

Microinjections
Transformation, Genetic
Gentavet
Gentacycol
Geneticin
Antibiotic G 418
Malaria
Malaria Vaccines
Anopheles gambiae
Genes, Bacterial

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
D A O'brochta, A M Handler
Proceedings of the National Academy of Sciences of the United States of America
N JasinskieneF H Collins
© 2020 Meta ULC. All rights reserved