Stable integration of a functional shRNA expression cassette into the murine leukemia virus genome

Virology
Katja Sliva, Barbara S Schnierle

Abstract

Short hairpin RNA (shRNA) can be stably expressed in cells to down-modulate gene expression. While retroviral and lentiviral vectors can be used to deliver shRNAs, the restricted viral titer is the major limitation for efficient gene transfer, which is especially important for cancer gene therapy. We were interested in using replicating murine leukemia virus (MLV) to enhance the shRNA transfer. Although stem loop structures could potentially interfere with the retroviral life cycle, we were able to demonstrate that the insertion of shRNA expression cassettes into MLV did not interfere significantly with viral fitness. The virus was genetically stable and able to silence target gene expression. Our results show that replicating MLVs are excellent tools for delivering shRNAs efficiently throughout the culture and have the potential to be used for gene function elucidation or even for cancer gene therapy.

References

Feb 19, 2000·Gene Therapy·R G VileN R Lemoine
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Aug 22, 2002·Current Cancer Drug Targets·I A J Lorimer
Nov 14, 2002·Annual Review of Genetics·Marcel TijstermanRonald H A Plasterk
Dec 21, 2002·Science·Jennifer Couzin
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wan-Xiang LiShou-Wei Ding
Mar 25, 2004·The Journal of General Virology·Etienne BucherMarcel Prins
Mar 25, 2004·The Journal of General Virology·M Otilia DelgadilloCarmen Simón-Mateo
Apr 14, 2004·Journal of Virology·Jennifer L Bromberg-WhiteSheri L Holmen
Dec 22, 2004·Virology Journal·Katja SlivaBarbara S Schnierle
Apr 23, 2005·Science·Charles-Henri LecellierOlivier Voinnet
May 17, 2005·Immunity·Yamina BennasserKuan-Teh Jeang

❮ Previous
Next ❯

Citations

Mar 3, 2007·Retrovirology·Pavlina KonstantinovaBen Berkhout
Sep 23, 2010·Virology Journal·Katja Sliva, Barbara S Schnierle
Aug 30, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Masayuki SanoJohn J Rossi
Apr 17, 2007·Advanced Drug Delivery Reviews·Lekha Dinesh Kumar, Alan R Clarke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.