Stable-Isotope Analysis of a Combined Nitrification-Denitrification Sustained by Thermophilic Methanotrophs under Low-Oxygen Conditions.

Applied and Environmental Microbiology
R PelK B Zwart

Abstract

To simulate growth conditions experienced by microbiota at O(inf2)-limited interfaces of organic matter in compost, an experimental system capable of maintaining dual limitations of oxygen and carbon for extended periods, i.e., a pO(inf2)-auxostat, has been used. (sup15)N tracer studies on thermophilic (53(deg)C) decomposition processes occurring in manure-straw aggregates showed the emission of dinitrogen gas from the reactor as a result of simultaneous nitrification and denitrification at low pO(inf2) values (0.1 to 2.0%, vol/vol). The N loss was confirmed by nitrogen budget studies of the system. Depending on the imposed pO(inf2), 0.6 to 1.4 mmol of N/day (i.e., 20 to 40% of input N) was emitted as N(inf2). When the pO(inf2) was raised, the rates of both nitrification and denitrification increased instantaneously, indicating that ammonia oxidation was limited by oxygen. In auxostats permanently running at pO(inf2) >= 2% (vol/vol), the free ammonium pool was almost completely oxidized and was converted to nitrite plus nitrate and N(inf2) gas. Labelling of the auxostat with [(sup13)C]carbonate was conducted to reveal whether nitrification was of autotrophic or heterotrophic origin. Incorporation of (sup13)CO(inf2) into populat...Continue Reading

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Citations

Sep 7, 2001·Annual Review of Microbiology·G A Kowalchuk, J R Stephen
Sep 26, 2001·Applied and Environmental Microbiology·M AlexandrinoA Lipski
Dec 7, 2005·FEMS Microbiology Ecology·Udo JäckelPeter Kämpfer
Nov 27, 2002·Applied and Environmental Microbiology·Anders R JohnsenPeter Roslev
May 1, 2002·FEMS Microbiology Ecology·H T S Boschker, J J Middelburg
May 10, 2017·Frontiers in Microbiology·Gonzalo V Gomez-SaezSolveig I Bühring
Oct 31, 1998·Applied and Environmental Microbiology·W R AbrahamO Pelz

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