Stable isotope dilution analysis of the Fusarium mycotoxins deoxynivalenol and 3-acetyldeoxynivalenol

Molecular Nutrition & Food Research
Michael BretzHans-Ulrich Humpf

Abstract

Trichothecenes are secondary metabolites produced by several fungi of the Fusarium genus during their growth period. They inhibit protein biosynthesis in eukaryotic cells resulting in numerous toxic effects such as diarrhea, vomiting, and gastro-intestinal inflammation. Considering its occurrence in food and feedstuff, deoxynivalenol (DON) is one of the most important trichothecenes. We report the synthesis of stable isotope labeled 15-d(1)-deoxynivalenol (15-d(1)-DON) from its natural precursor 3-acetyldeoxynivalenol (3-AcDON) as starting material. Furthermore, a method for the analysis of DON and 3-AcDON using HPLC-MS/MS with stable isotope labeled 15-d(1)-DON and 3-d(3)-AcDON as internal standards has been developed. In total, 18 cereal product samples were analyzed with contamination levels ranging from 10-301 microg/kg for DON and 5-14 microg/kg for 3-AcDON. This is the first report of an isotope dilution MS method for the analysis of type B-trichothecenes.

References

Jul 12, 2005·Analytical and Bioanalytical Chemistry·R KrskaE Welzig
Oct 18, 2005·Molecular Nutrition & Food Research·Michael BretzHans-Ulrich Humpf
Nov 10, 2005·Journal of Agricultural and Food Chemistry·Marianna KlötzelHans-Ulrich Humpf

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Citations

Dec 7, 2007·Analytical and Bioanalytical Chemistry·Michael Rychlik, Stefan Asam
Feb 2, 2012·Analytical and Bioanalytical Chemistry·Elisabeth VargaFranz Berthiller
Oct 2, 2007·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·V M T LattanzioA Visconti
Mar 12, 2015·Mycotoxin Research·Sebastian HickertHans-Ulrich Humpf
Jul 12, 2008·Molecular Nutrition & Food Research·Maika KönigsHans-Ulrich Humpf
Apr 15, 2011·Rapid Communications in Mass Spectrometry : RCM·Wei LiSusie Y Dai
Sep 7, 2016·Rapid Communications in Mass Spectrometry : RCM·Sebastian HickertHans-Ulrich Humpf
Feb 28, 2021·Mycotoxin Research·Jessica SchmidtHans-Ulrich Humpf
May 9, 2012·Journal of Chromatography. a·Vita Di StefanoKároly Vékey
Nov 12, 2013·Journal of Agricultural and Food Chemistry·Caroline WellThomas Hofmann
Oct 21, 2011·Journal of Agricultural and Food Chemistry·Qinghua WuHans-Ulrich Humpf
Sep 21, 2007·Journal of Agricultural and Food Chemistry·Benedikt CramerHans-Ulrich Humpf
Aug 17, 2006·Journal of Agricultural and Food Chemistry·Michael BretzHans-Ulrich Humpf

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