Stable isotope dilution ultra-high performance liquid chromatography-tandem mass spectrometry quantitative profiling of tryptophan-related neuroactive substances in human serum and cerebrospinal fluid

Journal of Chromatography. a
Eva HénykováMiroslav Strnad

Abstract

Many compounds related to L-tryptophan (L-TRP) have interesting biological or pharmacological activity, and their abnormal neurotransmission seems to be linked to a wide range of neurodegenerative and psychiatric diseases. A high-throughput method based on ultra-high performance liquid chromatography connected to electrospray tandem mass spectrometry (UHPLC-ESI-MS/MS) was developed for the quantitative analysis of L-TRP and 16 of its metabolites in human serum and cerebrospinal fluid (CSF), representing both major and minor routes of L-TRP catabolism. The combination of a fast LC gradient with selective tandem mass spectrometry enabled accurate analysis of almost 100 samples in 24h. The standard isotope dilution method was used for quantitative determination. The method's lower limits of quantification for serum and cerebrospinal fluid ranged from 0.05 to 15nmol/L and 0.3 to 45nmol/L, respectively. Analytical recoveries ranged from 10.4 to 218.1% for serum and 22.1 to 370.0% for CSF. The method's accuracy ranged from 82.4 to 128.5% for serum matrix and 90.7 to 127.7% for CSF matrix. All intra- and inter-day coefficients of variation were below 15%. These results demonstrate that the new method is capable of quantifying endogeno...Continue Reading

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Citations

Jun 8, 2017·Journal of Separation Science·Ilona SadokMagdalena Maria Staniszewska
May 12, 2020·Journal of Inherited Metabolic Disease·Lysiane BouletChristelle Corne
Jul 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Manuela CortesePiera Di Di Martino
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Aug 27, 2019·ACS Chemical Biology·Robert J TombariDavid E Olson
Jul 1, 2020·Combinatorial Chemistry & High Throughput Screening·Alexandrina Lungu-MoscaluJacobus F van Staden

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