Stable Isotope Labeling-Assisted Metabolite Probing for Emerging Contaminants in Plants

Analytical Chemistry
Qiuguo FuJay Gan

Abstract

Biotransformation is a notable modulator of the fate, bioaccumulation, and toxicity of contaminants in the environment. However, it is often formidable to identify unknown biotransformation products in the absence of reference standards, and this analytical challenge is particularly true for contaminants of emerging concern (CECs) that are mostly polar molecules without characteristic structures (e.g., Cl and Br) and in complex matrices such as plants. In this study, using the fibrate drug gemfibrozil as a model CEC and Arabidopsis thaliana as a model plant, we developed and demonstrated a novel analytical framework coupling deuterium stable isotope labeling with high-resolution mass spectrometry (SILAMS) in identifying plant biotransformation products. When exposed in A. thaliana cells, gemfibrozil was quickly taken up into the cells and extensively metabolized. The use of nonlabeled and deuterated gemfibrozil at a 3:1 ratio created unique diagnostic patterns in mass spectra, enabling the identification of 11 novel phase II amino acid/peptide conjugates. Similarity in mass fragmentation patterns and chromatographic behaviors was then employed to establish the probable structures. Two major metabolites were further confirmed as...Continue Reading

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Dec 26, 2016·Environmental Pollution·Qiuguo FuJay Gan

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Citations

Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eun-Ji WonKyung-Hoon Shin
Nov 15, 2019·Environmental Science & Technology·Qiuguo FuBenny Chefetz
Jul 1, 2020·Environmental Science & Technology·Weifang ChenGuibin Jiang

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