Stable isotope metabolic labeling with a novel N-enriched bacteria diet for improved proteomic analyses of mouse models for psychopathologies.

PloS One
Elisabeth FrankBoris Hambsch

Abstract

The identification of differentially regulated proteins in animal models of psychiatric diseases is essential for a comprehensive analysis of associated psychopathological processes. Mass spectrometry is the most relevant method for analyzing differences in protein expression of tissue and body fluid proteomes. However, standardization of sample handling and sample-to-sample variability are problematic. Stable isotope metabolic labeling of a proteome represents the gold standard for quantitative mass spectrometry analysis. The simultaneous processing of a mixture of labeled and unlabeled samples allows a sensitive and accurate comparative analysis between the respective proteomes. Here, we describe a cost-effective feeding protocol based on a newly developed (15)N bacteria diet based on Ralstonia eutropha protein, which was applied to a mouse model for trait anxiety. Tissue from (15)N-labeled vs. (14)N-unlabeled mice was examined by mass spectrometry and differences in the expression of glyoxalase-1 (GLO1) and histidine triad nucleotide binding protein 2 (Hint2) proteins were correlated with the animals' psychopathological behaviors for methodological validation and proof of concept, respectively. Additionally, phenotyping unra...Continue Reading

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Citations

Aug 16, 2014·Journal of Psychiatric Research·Michaela D FiliouChristoph W Turck
Aug 25, 2011·Molecular & Cellular Proteomics : MCP·Yaoyang ZhangChristoph W Turck
Feb 2, 2012·Expert Review of Proteomics·Daniel Martins-de-SouzaSabine Bahn
Nov 17, 2015·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Markus NussbaumerMichaela D Filiou
Jan 17, 2012·Proteomics·Michaela D FiliouChristoph W Turck
Aug 14, 2012·Proteomics·Michaela D FiliouChristoph W Turck
Feb 1, 2011·Proteomics. Clinical Applications·Michaela D FiliouDaniel Martins-de-Souza
Sep 18, 2015·Surface and Interface Analysis : SIA·M D FiliouC Lechene
Sep 4, 2015·Journal of Biosciences·Sumit SethiIshwar S Parhar
Mar 18, 2015·Molecular BioSystems·Michaela D FiliouGiuseppina Maccarrone
Dec 14, 2011·Trends in Plant Science·Borjana ArsovaWaltraud X Schulze
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Gabriel M SimonJeffrey I Gordon
Apr 12, 2012·Molecular & Cellular Proteomics : MCP·Christian TrötschelAnsgar Poetsch
Oct 21, 2016·Proteomics. Clinical Applications·Christoph W TurckMichaela D Filiou
Feb 27, 2016·Mass Spectrometry Reviews·Wolf D Lehmann
Apr 19, 2011·Analytical Chemistry·Xudong Yao
Aug 14, 2012·Journal of Psychiatric Research·Michaela D FiliouChristoph W Turck

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Methods Mentioned

BETA
PCR

Software Mentioned

ProRata
SEQUEST
MASCOT
QuantiSpec
BioWorks
SPSS

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