Stable isotope probing of hypoxic toluene degradation at the Siklós aquifer reveals prominent role of Rhodocyclaceae.

FEMS Microbiology Ecology
András TáncsicsTillmann Lueders

Abstract

The availability of oxygen is often a limiting factor for the degradation of aromatic hydrocarbons in subsurface environments. However, while both aerobic and anaerobic degraders have been intensively studied, degradation betwixt, under micro- or hypoxic conditions has rarely been addressed. It is speculated that in environments with limited, but sustained oxygen supply, such as in the vicinity of groundwater monitoring wells, hypoxic degradation may take place. A large diversity of subfamily I.2.C extradiol dioxygenase genes has been previously detected in a BTEX-contaminated aquifer in Hungary. Older literature suggests that such catabolic potentials could be associated to hypoxic degradation. Bacterial communities dominated by members of the Rhodocyclaceae were found, but the majority of the detected C23O genotypes could not be affiliated to any known bacterial degrader lineages. To address this, a stable isotope probing (SIP) incubation of site sediments with 13C7-toluene was performed under microoxic conditions. A combination of 16S rRNA gene amplicon sequencing and T-RFLP fingerprinting of C23O genes from SIP gradient fractions revealed the central role of degraders within the Rhodocyclaceae in hypoxic toluene degradation...Continue Reading

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Citations

Sep 22, 2018·FEMS Microbiology Ecology·Matthew Stott, Tillmann Lueders

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Datasets Mentioned

BETA
KY499472
KY499476

Methods Mentioned

BETA
amplicon sequencing
electrophoresis
PCR
DNA
chips

Software Mentioned

BLAST
cd
GREENGENES
GS Run Processor
blastn
hit
SILVAngs
est
OxyView
- PST3

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