Stable microfluidic flow focusing using hydrostatics

Biomicrofluidics
Vaskar GnyawaliScott S H Tsai

Abstract

We present a simple technique to generate stable hydrodynamically focused flows by driving the flow with hydrostatic pressure from liquid columns connected to the inlets of a microfluidic device. Importantly, we compare the focused flows generated by hydrostatic pressure and classical syringe pump driven flows and find that the stability of the hydrostatic pressure driven technique is significantly better than the stability achieved via syringe pumps, providing fluctuation-free focused flows that are suitable for sensitive microfluidic flow cytometry applications. We show that the degree of flow focusing with the hydrostatic method can be accurately controlled by the simple tuning of the liquid column heights. We anticipate that this approach to stable flow focusing will find many applications in microfluidic cytometry technologies.

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Citations

Nov 22, 2019·Chemical Society Reviews·Youchuang Chao, Ho Cheung Shum
May 28, 2020·Lab on a Chip·Cynthia RichardVictor J Cadarso
Aug 1, 2020·Molecular Reproduction and Development·Qinan Hu, Mariana F Wolfner
Oct 18, 2018·Molecular Biology of the Cell·Matthew R DilsaverDaniel L Levy
Dec 7, 2018·Biomicrofluidics·Tamer AbdelFatahSara Mahshid
Feb 9, 2019·Scientific Reports·Vaskar GnyawaliMichael C Kolios
Mar 20, 2019·Scientific Reports·Eric M StrohmMichael C Kolios
Jun 22, 2019·Journal of Colloid and Interface Science·Morteza JeyhaniScott S H Tsai
May 20, 2021·Lab on a Chip·Elisa M WassonMonica L Moya
Jun 5, 2021·Biomicrofluidics·Jyoti P GurungMatthew A B Baker
Feb 28, 2019··Lianne H. Y. SoAl Christopher De Leon

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