Stable multilineage xenogeneic replacement of definitive hematopoiesis in adult zebrafish

Scientific Reports
Isabell Hess, Thomas Boehm

Abstract

Bony fishes are the most numerous and phenotypically diverse group of vertebrates inhabiting our planet, making them an ideal target for identifying general principles of tissue development and function. However, lack of suitable experimental platforms prevents the exploitation of this rich source of natural phenotypic variation. Here, we use a zebrafish strain lacking definitive hematopoiesis for interspecific analysis of hematopoietic cell development. Without conditioning prior to transplantation, hematopoietic progenitor cells from goldfish stably engraft in adult zebrafish homozygous for the c-myb(I181N) mutation. However, in competitive repopulation experiments, zebrafish hematopoietic cells exhibit an advantage over their goldfish counterparts, possibly owing to subtle species-specific functional differences in hematopoietic microenvironments resulting from over 100 million years of independent evolution. Thus, our unique animal model provides an unprecedented opportunity to genetically and functionally disentangle universal and species-specific contributions of the microenvironment to hematopoietic progenitor cell maintenance and development.

References

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Jul 9, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nadeem YaqoobThorsten Schwerte
Sep 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Cristian Soza-RiedThomas Boehm
May 10, 2011·Methods in Cell Biology·David L Stachura, David Traver
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Isabell HessThomas Boehm
Aug 7, 2013·Blood Cells, Molecules & Diseases·Sonja BoatmanLeonard I Zon
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Citations

Oct 9, 2018·Frontiers in Cell and Developmental Biology·Jeremy T Baeten, Jill L O de Jong
Jan 5, 2021·Frontiers in Cell and Developmental Biology·Ramy ElsaidAna Cumano
Jun 26, 2020·IScience·Norimasa IwanamiThomas Boehm

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Datasets Mentioned

BETA
U71093.1
EF186007.3
BC129208
AF169032

Methods Mentioned

BETA
transgenic
flow cytometry
FCS
Flow
Assay
genotyping

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