Stable RNA interference of host thrombospondin-1 blocks Trypanosoma cruzi infection

FEBS Letters
Kaneatra J SimmonsF Villalta

Abstract

Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transcripts and protein expression in mammalian cells causing inhibition of T. cruzi infection. Addition of TSP-1 to these cells restores infection. Thus, host TSP-1, regulated by the parasite, plays a crucial role in early infection. This is the first report showing that a human parasite modulates TSP-1 expression to facilitate infection.

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Citations

Mar 14, 2009·Parasitology Research·Fernando VillaltaHerbert B Tanowitz
Feb 2, 2013·The Journal of Infectious Diseases·Fernando VillaltaGalina I Lepesheva
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Fernando VillaltaMaria F Lima
Mar 7, 2007·Cytokine & Growth Factor Reviews·Wilson SavinoSusana Gea
Nov 28, 2013·Pharmaceuticals·Olivia PerwitasariRalph A Tripp
Sep 4, 2007·Reviews in Medical Virology·Luis Isamu Barros KanzakiEnrique R Argañaraz
Feb 7, 2020·Developmental and Comparative Immunology·Lauren E FuessLaura D Mydlarz

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