Stable synaptic retention of serine-880-phosphorylated GluR2 in hippocampal neurons.

Molecular and Cellular Neurosciences
B StatesE B Ziff

Abstract

Phosphorylation of S880 within the GluR2 C-terminus has been reported to promote endocytosis of AMPA receptors (AMPARs) by preventing GluR2 interaction with the putative synaptic anchoring proteins GRIP and ABP. It is not yet established however, whether S880 phosphorylation induces removal of AMPARs from synaptic sites, and the trafficking of phosphorylated GluR2 subunits with surface and endocytosed GluR2 has not been directly compared within the same intact neurons. Here we show that phosphorylation of GluR2 subunits by PKC activated with phorbol esters is compartmentally restricted to receptors located at the cell surface. Endogenous AMPARs containing S880-phosphorylated GluR2 remained highly synaptic and colocalized with postsynaptic markers to the same extent as AMPARs which did not contain S880-phosphorylated GluR2. Moreover, following S880 phosphorylation, exogenous GluR2 homomers were found specifically at the cell surface and did not co-traffic with the internalized endosomal GluR2 population. We also show that GluR2 is endogenously phosphorylated by a constitutively active kinase pharmacologically related to PKC, and this phosphorylation is opposed by the protein phosphatase PP1. Our results demonstrate a population ...Continue Reading

Citations

Aug 31, 2010·Nature Neuroscience·Danielle M EversDaniel T S Pak
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
May 12, 2010·American Journal of Physiology. Cell Physiology·Lei XueJian Shi
Dec 6, 2012·Stroke; a Journal of Cerebral Circulation·Fan ZhangBingren Hu
Jul 5, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benjamin A SiddowayHouhui Xia
Jan 20, 2010·Molecular and Cellular Neurosciences·Charu MisraEdward B Ziff
Jun 3, 2020·The Journal of Experimental Medicine·Marcin BączykDaniel Zytnicki
Apr 3, 2019·Cellular and Molecular Life Sciences : CMLS·Diane BissenAmparo Acker-Palmer
Jan 24, 2012·Biochemical and Biophysical Research Communications·Mohammed FarooqPeter H Frederikse
Jul 2, 2010·Learning & Memory·Johannes GräffIsabelle M Mansuy

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