Stable transfection of Eimeria tenella: constitutive expression of the YFP-YFP molecule throughout the life cycle.

International Journal for Parasitology
Wenchao YanX Suo

Abstract

The obligate intracellular apicomplexan parasite Eimeria tenella, one of seven species of Eimeria that infect chickens, elicits protective cell-mediated immunity against challenge infection. For this reason, recombinant E. tenella parasites could be utilised as an effective vaccine vehicle for expressing foreign antigens and inducing immunity against heterologous intracellular microbes. A stable line of E. tenella expressing foreign genes is a prerequisite, and in this work an in vivo stable transfection system has been developed for this parasite using restriction enzyme-mediated integration (REMI). Two transgenic populations of E. tenella have been obtained that express YFP-YFP constitutively throughout the parasite life cycle. Southern blotting and plasmid rescue analyses show that the introduced exogenous DNA was integrated at random into the parasite genome. Although the life cycle of the transgenic populations was delayed by at least 12h and the output of oocysts was reduced 4-fold relative to the parental BJ strain of E. tenella, the transgenic parasites were sufficiently immunogenic to protect chickens against challenge with either transgenic or parental parasites. These results are encouraging for the development of tr...Continue Reading

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Citations

Feb 2, 2013·Parasitology Research·Guangwen YinXun Suo
Oct 31, 2012·Journal of Biosciences·Emily L Clark, Damer P Blake
Jun 16, 2009·Parasitology·V McDonald, M W Shirley
Jul 19, 2013·Journal of Parasitology Research·Hong ChenMichael Kurth
Nov 19, 2013·Trends in Parasitology·Damer P Blake, Fiona M Tomley
Sep 22, 2012·Trends in Parasitology·Ben J WoodcroftStuart A Ralph
Apr 22, 2009·International Journal for Parasitology·Jun ZouXun Suo
May 20, 2015·Veterinary Parasitology·Damer P Blake
Sep 25, 2012·International Journal for Parasitology·Joachim Müller, Andrew Hemphill
Aug 25, 2016·Parasites & Vectors·Virginia Marugan-HernandezFiona M Tomley
Apr 21, 2012·Avian Pathology : Journal of the W.V.P.A·M W Shirley, H S Lillehoj
Feb 28, 2019·Current Protocols in Microbiology·Iván Pastor-FernándezVirginia Marugán-Hernández
Feb 26, 2020·Infection and Immunity·Xinming TangXun Suo
Oct 19, 2017·Parasites & Vectors·Virginia Marugan-HernandezFiona M Tomley
Aug 31, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaoxi HuangXun Suo
Sep 13, 2017·International Journal for Parasitology·C E SuarezB M Cooke
Feb 23, 2021·Frontiers in Cellular and Infection Microbiology·Pauline GaboriaudFrançoise I Bussière

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