PMID: 1631052Jul 1, 1992

Stable transformation of a mosquito cell line results in extraordinarily high copy numbers of the plasmid

Proceedings of the National Academy of Sciences of the United States of America
T J MonroeB J Beaty

Abstract

Stable incorporation of high copy numbers (greater than 10,000 per cell) of a plasmid vector containing a gene conferring resistance to the antibiotic hygromycin was achieved in a cell line derived from the Aedes albopictus mosquito. Plasmid sequences were readily observed by ethidium bromide staining of cellular DNA after restriction endonuclease digestion and agarose gel electrophoresis. The plasmid was demonstrated by in situ hybridization to be present in large arrays integrated in metaphase chromosomes and in minute and double-minute replicating elements. In one subclone, approximately 60,000 copies of the plasmid were organized in a large array that resembles a chromosome, morphologically and in the segregation of its chromatids during anaphase. The original as well as modified versions of the plasmid were rescued by transformation of Escherichia coli using total cellular DNA. Southern blot analyses of recovered plasmids indicate the presence of mosquito-derived sequences.

References

May 2, 1996·Biochimica Et Biophysica Acta·T KobayashiJ H Collins
Dec 1, 1993·Insect Biochemistry and Molecular Biology·F A Shotkoski, A M Fallon
Sep 27, 2000·Biochimica Et Biophysica Acta·X ZhaoJ H Collins
Mar 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C C Wu, A M Fallon
Nov 14, 1997·Current Opinion in Biotechnology·L McCarroll, L A King
Mar 10, 1999·Insect Biochemistry and Molecular Biology·Z H Wang, A M Fallon
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T MatsubaraJ C Burns
Jan 1, 1992·Insect Molecular Biology·M J KovachB J Beaty
Dec 23, 1998·Environmental Health Perspectives·B J BeatyA Rayms-Keller
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·F CatterucciaT G Loukeris
Mar 23, 2010·Journal of Insect Physiology·A M Fallon, A Gerenday
Jul 1, 2006·Radiation Research·Takamitsu A KatoJ S Bedford
Feb 2, 1999·Insect Molecular Biology·Y G Zhao, P Eggleston

Citations

Sep 3, 1976·Science·H Weintraub, M Groudine
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G HadlaczkyV Tubak
Jan 1, 1990·Archives of Insect Biochemistry and Physiology·F A Shotkoski, A M Fallon
Jan 1, 1989·Medical and Veterinary Entomology·A C MorrisJ M Crampton
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B A HamkaloR T Schimke
Oct 31, 1985·The New England Journal of Medicine·R C SeegerD Hammond
Oct 22, 1982·Science·G M Rubin, A C Spradling
Jul 25, 1986·Science·D M ShahR T Fraley

Related Concepts

Hygromycin A
Shuttle Vectors
Transfection
Hygromycin B
Chromosomes
Genomic Hybridization
DNA Replication
Cinnamates
Restriction Mapping
Aedes

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