Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in vivo

The Plant Cell
C ForreiterL Nover

Abstract

Using Agrobacterium, we developed a method to transform an Arabidopsis cell suspension culture. A stably transformed cell line expressing high levels of firefly luciferase (Luc) was used for in vivo studies of thermal denaturation and renaturation of the enzyme and the protective role of different chaperones. Luc activity was monitored under heat stress and recovery conditions in control, thermotolerant cells and cells expressing plant chaperones after transient cotransformation with plasmids encoding proteins of the heat shock protein Hsp90, Hsp70, or Hsp20 family. The effects of the expressed proteins were specific. The Hsp17.6 class I protein maintained Luc activity on a level comparable with that observed in thermotolerant cells and improved Luc renaturation. Although transient expression of Hsp90 did not protect Luc from thermal denaturation, it accelerated Luc renaturation during recovery. In contrast to the other chaperones tested, overexpression of Hsp70 alone had no effect on denaturation and renaturation of Luc but enhanced Luc renaturation if coexpressed with Hsp17.6.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Horwitz
Dec 1, 1986·Archives of Biochemistry and Biophysics·J Feierabend, S Engel
Nov 26, 1984·Nucleic Acids Research·M Bevan
May 5, 1995·The Journal of Biological Chemistry·G J LeeE Vierling
May 1, 1994·Trends in Biochemical Sciences·U Jakob, J Buchner
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Feb 1, 1996·Current Biology : CB·J Rassow, N Pfanner
Dec 6, 1996·Science·S BoseJ Buchner
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·C SchneiderF U Hartl
Jan 1, 1997·Cellular and Molecular Life Sciences : CMLS·L Nover, K D Scharf
Jul 25, 1997·Cell·J L Johnson, E A Craig

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Citations

Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·J F Martínez-GarcíaP H Quail
Sep 21, 2000·The Plant Journal : for Cell and Molecular Biology·P SmýkalV Zárský
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Dongsun CaoChi-Lien Cheng
May 16, 2002·Plant Physiology·Concepción AlmogueraJuan Jordano
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Melisa K WallAnthony Maxwell
Mar 29, 2008·Cell Stress & Chaperones·Masood SiddiqueKlaus-Dieter Scharf
Oct 17, 2013·Assay and Drug Development Technologies·Takrima SadikotGeorge A Vielhauer
May 7, 2005·FEBS Letters·Tomohiro UemuraKunio Takeyasu
Jan 22, 2009·Plant, Cell & Environment·Joanna TrippKlaus-Dieter Scharf
Sep 29, 1998·Biochemical Pharmacology·T Scheibel, J Buchner
Jan 1, 2012·Computational and Structural Biotechnology Journal·Mikhail I Koksharov, Natalia N Ugarova
Feb 3, 2007·Molecular & Cellular Proteomics : MCP·Rudy MaorKen Shirasu
Apr 12, 2007·Molecular & Cellular Proteomics : MCP·Jelle Van LeeneGeert De Jaeger
Jan 30, 2008·Current Protocols in Cell Biology·Federica BrandizziChris Hawes
Jan 4, 2020·Analytical and Bioanalytical Chemistry·Zohreh JahangirizadehSaman Hossienkhani

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