PMID: 11607112Nov 1, 1990Paper

Stable transformation of plastids in higher plants

Proceedings of the National Academy of Sciences of the United States of America
Z SvabP Maliga

Abstract

Stable genetic transformation of the plastid genome is reported in a higher plant, Nicotiana tabacum. Plastid transformation was obtained after bombardment of leaves with tungsten particles coated with pZS148 plasmid DNA. Plasmid pZS148 (9.6 kilobases) contains a 3.7-kilobase plastid DNA fragment encoding the 16S rRNA. In the 16S rRNA-encoding DNA (rDNA) a spectinomycin resistance mutation is flanked on the 5' side by a streptomycin resistance mutation and on the 3' side by a Pst I site generated by ligating an oligonucleotide in the intergenic region. Transgenic lines were selected by spectinomycin resistance and distinguished from spontaneous mutants by the flanking, cotransformed streptomycin resistance and Pst I markers. Regenerated plants are homoplasmic for the spectinomycin resistance and the Pst I markers and heteroplasmic for the unselected streptomycin resistance trait. Transgenic plastid traits are transmitted to the seed progeny. The transgenic plastid genomes are products of a multistep process, involving DNA recombination, copy correction, and sorting out of plastid DNA copies.

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Citations

Dec 1, 1990·Plant Molecular Biology·G N YeJ C Sanford
Oct 1, 1993·Molecular & General Genetics : MGG·H CarrerP Maliga
Nov 1, 1991·Molecular & General Genetics : MGG·S M NewmanJ E Boynton
Jul 14, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Brigitta DudasPal Maliga
Sep 4, 2009·Plant Cell Reports·Asako SigenoHiroshi Yamagishi
Dec 31, 2009·Planta·Ezequiel Matías LentzFernando Félix Bravo-Almonacid
Mar 18, 2009·Molecular Genetics and Genomics : MGG·Daniela AhlertRalph Bock
Mar 8, 2012·Protoplasma·Jelli Venkatesh, Se Won Park
Dec 18, 2004·Plant Molecular Biology·Nathalie DufourmantelGhislaine Tissot
Oct 22, 2005·Plant Molecular Biology·Cilia L C LeliveltJacqueline M Nugent
Apr 24, 2009·Plant Molecular Biology·Sugey Ramona Sinagawa-GarcíaPal Maliga
Aug 20, 2010·Plant Molecular Biology·Weimin LiRalph Bock
Aug 1, 2007·Photosynthesis Research·Archie R Portis, Martin A J Parry
Jan 28, 2005·Transgenic Research·Mikhajlo K ZubkotAnil Day
Apr 11, 2006·Transgenic Research·Hirosuke KanamotoKen-Ichi Tomizawa
Jun 30, 2009·Transgenic Research·A K SinghK C Bansal
Jan 1, 1991·Biotechnology Advances·J H Oard
Jan 1, 1995·Biotechnology Advances·P VainY Henry
Oct 1, 1993·Current Opinion in Biotechnology·T M Klein, S Fitzpatrick-McElligott
Dec 1, 1990·Trends in Biochemical Sciences·R A Butow, T D Fox
Mar 15, 2005·Cell Biology International·Ekaterina SytnikNikolay Kuchuk
Jun 5, 2003·Archives of Biochemistry and Biophysics·T John Andrews, Spencer M Whitney
Jun 5, 2003·Archives of Biochemistry and Biophysics·Inger Andersson, Thomas C Taylor

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