Stable transformation of Spirulina (Arthrospira) platensis: a promising microalga for production of edible vaccines

Applied Microbiology and Biotechnology
Jaber DehghaniYadollah Omidi

Abstract

The planktonic blue-green microalga Spirulina (Arthrospira) platensis possesses important features (e.g., high protein and vital lipids contents as well as essential vitamins) and can be consumed by humans and animals. Accordingly, this microalga gained growing attention as a new platform for producing edible-based pharmaceutical proteins. However, there are limited successful strategies for the transformation of S. platensis, in part because of an efficient expression of strong endonucleases in its cytoplasm. In the current work, as a pilot step for the expression of therapeutic proteins, an Agrobacterium-based system was established to transfer gfp:gus and hygromycin resistance (hygr) genes into the genome of S. platensis. The presence of acetosyringone in the transfection medium significantly reduced the transformation efficiency. The PCR and real-time RT-PCR data confirmed the successful integration and transcription of the genes. Flow cytometry and β-glucuronidase (GUS) activity experiments confirmed the successful production of GFP and the enzyme. Moreover, the western blot analysis showed a ~ 90 kDa band in the transformed cells, indicating the successful production of the GFP:GUS protein. Three months after the transfor...Continue Reading

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Citations

Jul 28, 2020·Biotechnology and Bioengineering·Bishal KhatiwadaHelena Nevalainen
Dec 22, 2019·Biotechnology Advances·Jaber DehghaniYadollah Omidi
May 4, 2021·Frontiers in Microbiology·McKenna HicksDavid L Bernick
May 9, 2021·Physiologia Plantarum·Konstantinos VavitsasKarin Stensjö

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Methods Mentioned

BETA
PCR
electrophoresis
flow cytometry
flow
transfection

Software Mentioned

SnapGene Viewer

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