Stachydrine, a major constituent of the Chinese herb leonurus heterophyllus sweet, ameliorates human umbilical vein endothelial cells injury induced by anoxia-reoxygenation

The American Journal of Chinese Medicine
Jun YinYou-Jin Shen

Abstract

Stachydrine is a major constituent of Chinese herb leonurus heterophyllus sweet, which is used in clinics to promote blood circulation and dispel blood stasis. Our study aimed to investigate the role of stachydrine in human umbilical vein endothelial cells (HUVECs) injury induced by anoxia-reoxygenation. Cultured HUVECs were divided randomly into control group, anoxia-reoxygenation (A/R) group and 4 A/R+stachydrine groups. HUVECs in the control group were exposed to normoxia for 5 hours, while in all A/R groups, HUVECs underwent 3 hours anoxia followed by 2 hours reoxygenation, and HUVECs in the 4 A/R+stachydrine groups were treated with 10(-8) M, 10(-7) M, 10(-6) M and 10(-5) M (final concentration) of stachydrine respectively. After anoxia-reoxygenation, tissue factor (TF) was over-expressed, cell viability and the concentrations of SOD, GSH-PX and NO were declined, while LDH, MDA and ET-1 were over-produced (p < 0.05 to 0.001 vs. the control group). However, in stachydrine treated groups, TF expression was inhibited at both mRNA and protein levels, while the declined cell viability and SOD, GSH-PX, NO as well as the enhanced LDH, MDA and ET-1 levels occurred during anoxia-reoxygenation were ameliorated and reversed effective...Continue Reading

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Citations

Oct 19, 2011·Clinical and Experimental Pharmacology & Physiology·Xin H LiuYi Z Zhu
Apr 8, 2014·Fitoterapia·Kenny KuchtaHans Wilhelm Rauwald
Aug 21, 2015·Journal of Ethnopharmacology·Baudry N NsualaAlvaro Viljoen
Jun 8, 2013·Journal of Cellular Biochemistry·Luigi ServilloMaria Luisa Balestrieri
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Apr 9, 2011·The American Journal of Chinese Medicine·Zhi-Ye YangZhi Chao
Apr 1, 2016·Journal of Food and Drug Analysis·Hsin-Yi Hung, Tian-Shung Wu
Apr 15, 2015·Journal of the American Chemical Society·Fang-Yuan ChangSean F Brady

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